reference protein 中文意思是什麼

reference protein 解釋
參比蛋白
  • reference : n 1 （對委員、審查人等的）委託；委託項目[范圍]。2 說到，論到，提到。3 參考；參考書；附註，引證；...
  • protein : n. 【化學】朊，蛋白（質）。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Mass spectrometry technique will play more and more role in the field of sequence analysis. standard amino acid thiohydantoins are required as reference standard for development of c - terminal protein sequencing based on the thiohydantoin procedure

    在蛋白質c端（異）硫氰酸法順序測定技術中，標準氨基酸乙內酰硫脲（ th - aa ）的制備是必須首先要解決的問題。
  3. Caseins and caseinates. determination of protein content reference method

    酪蛋白和酪朊酸鹽.蛋白質含量的測定
  4. Determination of protein content of caseins and caseinates ; reference method

    乾酪素和酪朊酸鹽蛋白質含量的測定.參考法
  5. Following the purification of the expressed protein, a elisa was developed to detect reference antisera of h7, h5 and h9 subtypes. the result indicated that h7 antisera was positive, h5 and h9 antisera were negative

    用電洗脫方法純化菌體表達ha1蛋白后，建立了間接elisa方法，並對aivh7 、 h9 、 h5亞型血清進行檢測，結果h7亞型血清呈陽性， h5 、 h9亞型血清呈陰性反應。
  6. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  7. Haemostaseology - determination of protein c activity - reference measurement procedure with a chromogenic peptide substrate

    止血學. c蛋白活性的測定.肽基色譜參照測量程序
  8. Finally digestive enzyme activities in hepatopancreas of e. sinensis fed on experiment diets with different protein level during juvenile and ovarian maturation. the purpose is to accumulate basal information for reproductive nutrition, and provide reference for breeding broodstock in seed production and formulation of broodstock diet

    此外，還研究了蛋白質營養對中華絨鰲蟹卵巢發育期和仔蟹生長期消化酶的影響，以期為中華絨鰲蟹的生殖營養生理研究積累基礎資料，同時也為人工育苗生產過程中的規范親體培育及全價配合飼料的研製提供理論依據。
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