replacement vector 中文意思是什麼
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To clone large and random dna fragments, the second generation of yac, pjs97 - pjs98 was modified as gene replacement vector in streptomyces
4kb和1 . okb片段，而且還插入了勿，七刃j和spc心t廠抗性基因。 -
For desired gene replacement, hyg / cml and str / spc gene cassettes were also incorporated into the new vector system. it is expected that hygwould be integ
為了驗證新型yac載體系統的功能，我們將phz621 ~ phz622進行了適當地改造，構建了質粒phz625 。 -
In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts
我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。 -
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces
為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。 -
Secondly another vector phz1117 which for replacement of sc ( j11. 17 / scd78. 01, was constructed. phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained
其次，構建了另一個用於置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3並通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。 -
Phz621 - phz622, the new gene replacement vector system, had been constructed through the insertion of the 1. 0kb and 1. 4kb flanking sequences of hau3r gene from wild - type s. lividans in the same natural orientation
預期在攜帶有大插入片段的基因置換yac分子進入野生型變鉛青鏈黴菌后， yac分子將通過1 . 4kb或1 -
Prior to the vector construction, the availability of gene replacement was extensively tested in streptomyces. sp. fr - 008
在載體構建之前，我們先在鏈黴菌fr - 008中驗證了基因置換方法的可行性。 -
The total dna of exoconjugants acquired dna degradation phenotype during electrophoresis, which suggested that the dnd gene cluster was heterologously expressed. the phz1358 was used sucessfully as a vector for gene replacement to identify the biosynthetic pathway genes for nanchangmycin in s. nanchangensis ns3226. however, the gene replacement in another potential pks cluster has not succeeded till now
將利用phz1358構建的基因置換結構（孫宇暉，未發表）導入南昌鏈黴菌ns3226中進行了基因置換實驗，協助完成了南昌黴素生物合成基因簇的克隆，但對另一潛在pks基因簇的基因置換實驗目前還未能完成。
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