reporter gene 中文意思是什麼

reporter gene 解釋
報道基因，報告基因[處于另一基因下游並可反映轉錄及上游基因表達水平的基因
  • reporter : n. 報告者；呈報者；采訪記者，新聞通訊員；審判[議事]記錄員；指示器。adj. -torial
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Development of a functional reporter gene assay for the identification of glucagon - like peptide - 1 receptor agonist

    1受體激動劑功能性報告基因分析系統的構建
  2. These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis

    （ 3 ）兩個基因在反義植株里的表達都受到不同程度的抑制。在反義leetr1植株里， leetr1表達被抑制后， leetr2的表達也受到不同程度的抑制。同時，反義leetr2植株里也有相似的結果。
  3. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  4. Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

    選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg
  5. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  6. Improvement of genetic transformation system by using gfp as reporter gene on pepper

    應用綠色熒光蛋白報告基因優化辣椒的遺傳轉化體系
  7. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  8. Cloning and identification of nf - b p50 rel homology domain and detection of its autonomous reporter gene activity

    50亞基同源結構域的克隆鑒定及自主報告基因活性的檢測
  9. At the same time, we transfered chs to quamocliy pennata by agrobacterium mediated and established genetic transformation system of quamocliy pennata through the guide of gus reporter gene

    並以農桿菌介導將chs導入蔦蘿中，初步建立了蔦蘿的遺傳轉化系統，為今後蔦蘿遺傳改良的進一步研究奠定基礎。
  10. So we can say that the red gene may also be used as a reporter gene

    該重組菌株既能產生幾丁質酶，又能使菌體在紫外線下呈現紅色熒光。
  11. Our interesting is to use the red gene as a reporter gene

    為驗證redghe能否用來作為分子生物學研究的報告基因，將克隆在clon 。
  12. Based on the foundation research of the interaction of virus and host actin, we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster, a gfp - actin fusion gene was obtained

    本文在總結前人關于病毒與宿主肌動蛋白相互作用的研究的基礎上，利用綠色熒光蛋白基因為標記基因，與果蠅肌動蛋白基因5cactin基因融合，構成gfp - actin融合基因。
  13. Analysis of transcriptional activation abilities of three gmdreb proteins in yeast indicated that gmdreba and gmdrebb could activate the expression of a reporter gene, whereas gmdrebc could not

    轉錄激活活性分析表明， gmdreba和gmdrebb蛋白能夠激活下游報告基因的表達，而gmdrebc不能，說明gmdrebc蛋白不具有轉錄激活活性。
  14. Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants

    對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。
  15. ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling

    （ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。
  16. A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19

    進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因，構建了大腸桿菌啟動子探針載體phn1005 ，該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度；其3 』端含rrnat1t2終止子，可允許克隆強啟動子；在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀； gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止，可保證gfpmut3以正確的讀碼框翻譯。
  17. Standard guide for measuring the presence of planar organic compounds which induce cyp1a, using reporter gene test systems

    用指示器基因測試系統測定感應cyp1a的共面有機化合物的存在的標準指南
  18. Reporter gene is very important for molecular biology. until now more than 99 % microorganisms in the nature have not been cultured

    自然界的微生物中約99是目前還不能培養的，這些未能培養的微生物蘊藏著極其巨大的基因資源，是篩選有用基因的重要來源。
  19. A novel reporter gene, green fluorescent protein gene ( gfp ) has been widely used in study on gene expression and protein localization among living organisms

    主要研究結果如下： 1考查了廣宿主范圍穩定性質粒ptr102應用於華癸中生根瘤菌分子生物學研究的可行性。
  20. In transgenic tobacco plants, the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1. 2 kb pdf1. 2 promoter fused to a gus reporter gene was induced by meja

    （ 1 ）從擬南芥基因組中擴增出長度約為1200bp的pdf1 . 2啟動子，與gus構建的融合基因在煙草中的表達受meja誘導。
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