resistance assay 中文意思是什麼

Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment

用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用，並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平， mcf - 7 adr呈強陽性，而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % ， mcf - 7細胞系細胞陽性率為1 . 8 % 。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Application of rapid detection for rifampin resistance in clinical isolates of mycobacterium tuberculosis by phage amplified biologically assay

等位基因特異性多重聚合酶鏈反應方法用於快速檢測結核分枝桿菌利福平耐藥株

Leaf disc assay for identification of resistance of strawberry to powdery mildew and screening of fungicides

草莓抗白粉病的離體鑒定及農藥的篩選

Finally, in order to investigate whether these two virus are resistant to complement attack, and whether the resistance were mediated by incorporating cellular complement regulators of cd55, cd59 into their out membrane, complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody

將這兩種病毒分別暴露於人血清補體后，發現在有cd55 、 cd59中和性抗體存在的情況下，補體溶解hiv病毒的效率明顯增加； eev病毒的感染活性在空斑實驗中也明顯降低，結果與這兩種蛋白在病毒表面（ eev ）存在的事實相符，提示病毒表面的cd55

Standard test method for determining the resistance of paint films and related coatings to fungal defacement by accelerated four - week agar plate assay

用加速的四周瓊脂平面培養分析測定漆膜及有關塗層耐黴菌損壞的標準試驗方法

In transgenic tobacco plants, the transient - expression assay of the chimeric gene ( 4 x gcc - 35s min : : gus ) demonstrated that the 4 x gcc - 35smin promoter could respond to meja treatment and the gcc box is an important element in response to ja signaling. moreover, this experiment results would be meaningful to improve the crops characterization of resistance against various environment stresses or to study the regulation of gene expression in transgenic plants

（ 3 ）以反向的4xgcc重復序列（ placzi 4xgcc （ 》作為placzi4xgcc的突變體， 6半乳糖苦酶活性分析的結果表明，與野生型的相比，突變的gcc元件不能與jerfi 2 3 4相互作用， p半乳糖苦酶實驗不能呈現出藍色反應；證明gccbox與jerf 2 3 4是特異性結合。

And pcr - rflp analysis was a sensitive, rapid and simple assay for the detection of nucleotide sequence change in ser 83. and our data sugget that pcr - rflp may be a useful device for detecting the mutation in gyra gene associated the resistance to fqns

本研究通過試驗條件的篩選，建立了pcr - rflp檢測豬源致病性沙門氏菌gyra基因點突變的方法，可用於沙門氏菌氟喹諾酮類耐藥性的分子水平檢測和流行病學監測。