restriction endonuclease 中文意思是什麼

restriction endonuclease 解釋
核酸限制性內切
  1. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  2. Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank, a universal primer set l15774 / hsf21 was used as positive control of the template quality, at the meantime, two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ), sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species. a musk deer ( moschus ) specific primer set wf / mr was designed, siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products

    經過對來自genbank中的15種鹿類動物的cytob基因序列的比較，用通用引物l15774和hsf21作為模板的質量控制，設計了特異性引物df dr和cf cr來鑒定馬鹿、梅花鹿、狍；設計了麝類特異性引物mf mr ，用限制性內切酶rsa酶切擴增產物來區分原麝和林麝。
  3. The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed

    本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。
  4. The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively

    結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。
  5. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  6. The recornbinant vector pcambia 1305. 1 containing s gene was confirmed by double digestion of restriction endonuclease

    經雙酶切鑒定證實s基因克隆到載體pcambia1305 . 1上。
  7. The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion

    轉化感受態大腸桿菌dh5 ，經pcr及限制性內切酶分析得到陽性克隆。
  8. 3. pbv220 - endostatin was transformed into e. coli dh5a. the positive colony was screened and identified by pcr and restriction endonuclease digestion

    3 .將重組質粒轉化到克隆菌dh5a中，經pcr篩選和限制性內切酶鑒定，得到陽性克隆菌株。
  9. E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2, and subcloned into the expression vector ppic9 ' s mcs, which was identified by enzyme digestion and pcr amplification

    將重組質粒t - e2的e2基因酶切后，亞克隆到表達載體ppic9上，經酶切和pcr鑒定，命名為ppic9 - e2 。
  10. The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease. agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method

    用三親交配法將重組克隆導入根癌農桿菌，獲得了含pbi121重組質粒的eha105農桿菌菌株。
  11. The interest gene was inserted in the - tha l. tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system. after analysis by restriction endonuclease and pcr, the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid, the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l

    同時將該目的基因插入到桿狀病毒表達系統的供體質粒pf _ （ ast ） b _ （ ac ） htb的xba 、 xho多克隆位點間，經酶切、 pcr鑒定后，將重組的供體質粒gp1 - fast轉化到含有桿狀病毒和輔助質粒的dh10b _ （ ac ）感受態細胞中，獲得了表達gpvh1株vp1的重組桿狀病毒gp1 - bac 。
  12. Based on the restriction endonuclease sites of the reference sequences of a, b, e subgroups published in ncbi, the dna was digested by restriction endonucleases bgiii, bamhi, sspi. the expected results were obtained when digested by bgiii specific to a subgroup or sspi specific to a and e subgroups

    結果發現，擴增到的目的帶只能用對a亞群特異的bgl限制酶或對a 、 e亞群特異的ssp限制酶切開，而不能用對b亞群特異的bamh限制酶切開。
  13. A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ). the cdna subtractive library after amplification included 100 blue clones and 400 white clones, 75 ones of which were selected to prepare for plasmid. identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector

    構建高消減效率的+ gz重復暴露大鼠腦cdna消減文庫，擴增后cdna文庫包含約400個白色克隆和100個藍色克隆，克隆飽滿清晰，隨機挑取75個白色克隆，制備質粒后，以ecor酶切分析，表明大部分克隆入質粒載體。
  14. 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector. it was identified by restriction endonuclease digest analysis, pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene

    擴增產物連接到pgem - teasy載體上，轉化入大腸桿菌jm109中進行藍白斑篩選后，用酶切、 pcr鑒定和測序的方法鑒定出重組陽性質粒（ pgem - hc和pgem - ha ） 。
  15. Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ), mapped for restriction endonuclease sites and an about 5. 0kb fragment was further subcloned and sequenced. through coding region specific primer, we amplied it ' s corresponding cdna, named st901. st901 is 2889bp long, contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp, 140bp, 39bp ) and two introns ( 472bp, 2s3bp ) in the coding region, encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda

    以同源探針篩選馬鈴薯基因組文庫，得到四倍體馬鈴薯基因組dna ? st901 ，基因全長2889bp ，含3個外顯子（長度分別為475bp ， 140bp ， 39bp ）和2個內含子（長度分別為472bp ， 253bp ） ； 5 』端含有1447bp的啟動子區段，該區段具備一般啟動子的基本元件tatabox和caatbox ； 3 』非編碼區長63bp ，具hind酶切位點，沒有發現保守的加尾信號。
  16. The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert

    經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中，再轉化大腸桿菌tgi感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  17. The specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resuctant construct contained the gene of interest f. the positive cloned was sequenced by sanger ' s dideoxy sequencing method. the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid

    經核苷酸測序，該基因含一個1662bp的開放閱讀框，編碼553個氨基酸，與國外報導的新城疫各毒株f基因相比，核苷酸序列同源性為88 . 3 96 . 4 ，氨基酸同源性為92 . 1 96 . 4 。
  18. Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease, pcr and nested pcr on the basis of the genetic sites of pbluebachisc, which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l

    純化該重組質粒並與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 ， 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明，獲得了prv - vp7基因與桿狀病毒dna的重組子，命名為a - 1代病毒。
  19. The amplified e2 fragments of two hcv strains were all 1280bp in length by 2 % agrose gel electrophoresis. expected size of 1280bp of 2 fragments and their specificity were confirmed by restriction endonuclease digestion and then they were cloned respectively into pmd - 18t vector

    以此病毒rna為模板，利用rt - pcr技術，擴增出了豬瘟野毒株hcv - jn 、 hcv - yc完整的e2基因，用pmd - 18t載體克隆，經電泳檢測、酶切分析及pcr鑒定初步證實了所擴增片段的特異性。
  20. The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation

    經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。
分享友人
分享到 Line

分享到臉書