rprv 中文意思是什麼
rprv
解釋
遙控研究機-
To differentiate the animals immunized with rprv - ha and those naturally infected with prv or sfv in future, two differentiating diagnostic methods were developed based on recombinant viral antigens
為了區分此重組病毒疫苗免疫的動物和自然感染prv和siv的動物，本項研究利用基因工程抗原建立了兩種分子鑒別診斷方法。 -
After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。 -
The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
結果表明所獲得的重組病毒（ rprvha ）遺傳性狀穩定，在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白，為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。 -
Minimum dose of protection of recombinant pseudorabies virus rprv - gp5 against pseudorabies in sheep
對偽狂犬病最小免疫劑量的測定
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