rt run time 中文意思是什麼

rt run time 解釋

Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken

結果12個參考毒株均能擴增出約679bp的目的片段，而陰性對照的常見5種雞病病原：雞新城疫病毒（ ndv ）、雞傳染性支氣管炎病毒（ ibv ）、雞傳染性貧血病毒（ caiv ）、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段；應用建立的技術對疑似ibd的34份臨床病料進行檢測，並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。