saci 中文意思是什麼

saci 解釋
非洲工商業公司
  1. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  2. And wibdv specific rt - pcr also uesed to detected these samples in the years from 1993 to 2000, there are 8 strains belong to wibdv and 13 belong cibdv. two strains ca n ' t typing for they can be cleaved with both of enzymes sspl and saci.

    結果發現1993年至2003年的病料或分離的毒株中，有8株確定為vvibdv ， 13株踴定為cibdv ，另外，有兩株不能確定。
  3. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  4. Two useful restriction endonucleases ( sspl and saci ) were choosed to type the different pathgenic ibdv strains. the result is saci only cleaved cibdv ( 4vaccine strains : bj836, b87, d78, bdc and 6 standard cibdv strains : hel, he2, he3, he4, sd3 / 98, zj1 / 98 ) and vibdv ( american variant - e ) rt - pcr products, whereas products obtained with vvibdv strains ( yl1, yl2, yl5, ylz ) were only cleaved with sspl

    選用具有分型意廬盧人聳2003屆碩十學位論文2義的兩種限制性內切酶（ saci和sspi ）建立的rea ，對5株屬于cibdv的疫苗株和6株標準強毒株；屬于vvibdv的毒株gx 、 yli 、 ylz和ylz等分離毒：屬于葉v的美國變異e株進行酶切分析，結果與前人的研究相符。
  5. But the vvidbv strain gx8 / 99 that had been identified by gui only have saci side, and no sspl, the reason need farther research. according to the specific different sequence between cibdv and vvibdv in vvp2 gene. we also designed two sets of primers ( cibda + cibds and vvibda and vvibds )

    另外，針對不同強弱毒株的1 v的pz高變區序列的差異：設計合成了vvibdv的型特異性引物（ ibda vvibds人建立型特異的rt pcr ，只需一次反應即可區分cibdv和vvibdv 。
  6. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  7. Dna band is about 600 bp when p1asmid was digested by sacii and saci enzyme, and is equal to that of hbsag gene ; but it is about 700bp when plasmid was digested by xhoi and saci enzyme, and is equa1 to the weight of s, . / hbsag fusion gene

    經sacll和saclk切電泳可見約600hp的dna帶，與hbsag基因大小相當；用xhol和sacl切電泳可見約700hp的dna帶，同s …與hbsag基因融合片段的大小相等說明s ； 。
  8. By the same method, the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion. after that, the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method. the pcr amplification indicated the lba4404 strain containning cpti gene. the lba4404 strain was used in genie transformation of mustard

    經酶切和pcr擴增驗證后，以xbai和saci雙酶切pgem ? cp和pbi121 ，將切下的cpti片段和pbi121載體片段連接構建成表達載體pbi121 - cp ，用凍融法導入農桿菌lba4404 ，提取質粒，經pcr擴增檢測，用於芥菜的遺傳轉化。
  9. Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ), mapped for restriction endonuclease sites and an about 5. 0kb fragment was further subcloned and sequenced. through coding region specific primer, we amplied it ' s corresponding cdna, named st901. st901 is 2889bp long, contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp, 140bp, 39bp ) and two introns ( 472bp, 2s3bp ) in the coding region, encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda

    以同源探針篩選馬鈴薯基因組文庫，得到四倍體馬鈴薯基因組dna ? st901 ，基因全長2889bp ，含3個外顯子（長度分別為475bp ， 140bp ， 39bp ）和2個內含子（長度分別為472bp ， 253bp ） ； 5 』端含有1447bp的啟動子區段，該區段具備一般啟動子的基本元件tatabox和caatbox ； 3 』非編碼區長63bp ，具hind酶切位點，沒有發現保守的加尾信號。
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