sandwich elisa 中文意思是什麼

sandwich elisa 解釋
夾心酶聯免疫分析
  • sandwich : n 三維治〈英國五港之一〉。n 三明治,夾心麵包片;三明治層狀結構[物]。 ride [sit] sandwich 夾坐二人...
  • elisa : 埃莉薩
  1. Detecting gp of hfrs virus with double antibodies sandwich elisa

    法檢測腎綜合征出血熱漢灘病毒糖膜蛋白
  2. A few years latter the research showed, vp2 hydrophilic region and antigen variant and virulence of ibdv had very affinity. in order to lucubrate difference of virulence and antigen, and validate correntness of sequencing result, the test adopted sandwich enzyme - liked immunosorbent assay ( elisa ) and antigen caprure - elisa to determine virulence and antigen. and validate correntness of above - mentioned result

    而近幾年的研究表明, vp2高變區與ibdv的抗原變異和毒力強弱有密切的關系,為進一步了解強弱毒株毒力和抗原的差別,並進一步證實測序結果的正確性,本試驗採用雙抗夾心elisa和ac - elisa的方法分別對其毒力和抗原進行了測定,並通過動物試驗證實了,其結果與序列分析結果基本一致。
  3. Cells were collected after being passaged 5 times and used to identify infection by rt - pcr, direct fluorescent antibody, sandwich elisa. results indicated that integrated particles of csfv could be obtained from constructed full - length cdna

    連續傳代5代以後,收集各w摘要代細胞至第10代,採用rt一pcr 、直接熒光抗體染色和夾心elisa技術進行鑒定,結果均為陽性。
  4. Sandwich elisa assay is a sensitive, specific and stable technique

    鞏乃a法檢測可溶性比卜工靈敏、特異、穩定; 2
  5. Preparation of monoclonal antibodies against troponin i and development of one - step sandwich elisa

    單克隆抗體制備及酶免疫法的建立
  6. Circulating antigen detection in schistosomiasis japonica patients by sandwich elisa using polyclonal and monoclonal antibodies

    法檢測日本血吸蟲病患者血清循環抗原
  7. Sandwich elisa is an immunoassay technique in common use, especially for the determination of macromolecular protein antigen

    雙抗體夾心elisa是常用的免疫分析技術,主要適用於檢測大分子的蛋白質抗原。
  8. Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1

    方法:以w6 32包被酶標板,捕捉樣品中可溶性hla ,加入一抗2m抗體,再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線: 1
  9. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后,隨機挑選20個噬菌體克隆, elisa鑒定出9個陽性克隆,經dna測序推出三種氨基酸序列: c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ,其中優勢克隆序列為c - rrpaqsg - c ;鑒定結果顯示陽性克隆能夠與tnf受體結合,並且能夠阻斷tnf與受體的結合,提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性,為tnfa表位模擬肽。
  10. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面: 1 、 tnf結合肽的研究:以rhtnf為靶對噬菌體環七肽庫進行篩選,以尋求可拮抗tnf活性的小分子短肽。
  11. Methods a double mono - clonal antibody sandwich enzyme - linked immunosorbent assay ( elisa ) was used to detect the circulating antigens, antibodies and immune complex in 109 sera of neurocysticercosis

    方法:用elisa法檢測109例腦囊尾蚴病患者血清抗原、抗體及循環免疫復合物。
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