secreted protein 中文意思是什麼
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Vaginal discharge, or leucorrhea, is the clear or semi - opaque protein - like substance secreted to keep the vagina moist and healthy. it should be odorless. many women find vaginal discharge annoying, but this is in fact normal
其實陰道分泌物是正常生理現象，正常情況下，婦女陰道會有一些清澈或半透明的無味蛋白狀分泌物，即白帶，主要為保持陰道濕潤健康。 -
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
Follistatin, a kind of secreted protein, is known to antagonise the function of several members of the tgf - beta ( transforming growth factor - beta ) superfamily including myostatin, the most powerful inhibitor of muscle growth characterised to date
卵泡抑素（ follistatin ）是一種分泌蛋白，它可以拮抗tgf - beta （ transforminggrowthfactor ，轉化生長因子）超家族中幾種成員的功能，包括myostatin 。 -
To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。 -
The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production
應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。 -
Recovery estimated from the safh4 plant line indicates that 9 ( jl g of pure active scfv can be obtained per gram of fresh leaf material, on a laboratory scale. the production of the scfv antibody proteins in plant root exudates was also addressed. the scfv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g - 1 dry weight of root day - 1
在水培條件下，轉基因煙草根可連續分泌具有活性的重組抗乙肝病毒表面抗原pres1 （ 20 - 47 ）單鏈抗體進入到液體培養液中，不須破壞植物即可連續獲得重組單鏈抗體，為利用植物生物反應器連續生產單鏈抗體開辟了新途徑。 -
Introduction bovine seminal plasma protein family secreted by the seminal vesicles. bovine seminal plasma is the major component of bovine seminal plasma
目的牛精漿（ bovineseminalplasma ）作為精子的載體，是牛睪丸、附睪及附性腺分泌的一種復合物。 -
Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。 -
Animal growth hormone ( gh ) is a very important protein hormone which is secreted by the anterior pituitary cells and plays many important roles on animals, such as regulating somatic growth and stimulating appetite, affecting various metabolic activities and improving the food transformation rate, etc. although gh has so many functions, it is limited in the application and functional researches because the natural growth hormone content is very low and difficult to purify
動物生長激素（ growthhormone ， gh ）是由腦垂體間葉細胞分泌的一種多肽激素，具有調節生長發育，參與體內代謝調節，增進食慾，提高食物轉化率等作用，然而垂體中生長激素含量極低，分離純化比較困難，使得生長激素生理功能的進一步研究和應用受到限制。 -
Further study showed that aii protein was a kind of non - secreted protein, and aii gene was located on the chromosome of b. thuringiensis or b. cereus
研究中還發現，蘇雲金芽胞桿菌aii蛋白是一種非分泌型蛋白。 -
The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。 -
The expression products of pel gene was analysis by sds - page and olive oil plate, and the result indicated that pel gene was functionally overexpressed in pichia pastoris and up to 95 % of the secreted protein
表達蛋白pel - gs分泌至培養基中，分子量約28kd ，與pel大小相近，佔分泌蛋白的95 。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine. multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol. the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified
轉化嗜甲醇酵母獲得耐受4mg mlg418的菌株，經sds - page電泳和westernblotting檢測表明其上清培養液中的蛋白可與猴抗pzp3抗體和兔抗hcg抗體發生特異性的反應。 -
In summary, we have expressed secreted mpt64 protein of mtb by molecular biology technique. vaccination with recombinant mpt64 protein is an effective means to genenrate humoral immunity renponses and protec
狀1wi述，木研究渺過分于丫物學技術杉叢m丁b分泌鉆i 」 mpt64上門。 -
Secreted expression of mature peptide gene of human bone morphogenic protein - 7 in pichia pastoris
7成熟肽基因在巴斯德畢氏酵母中的分泌表達 -
It is quite easy to modify genes so that their encoded protein will be secreted, and there are also techniques for targeting proteins in the circulation to particular organs
很容易修飾基因，使它們所編碼的蛋白質能被分泌，也有技術使血液循環中的蛋白質打靶到特殊器官。 -
After inducement by methanol, the soluble part of trail was secreted into supernatant. a high - level strain named as gtr48 with more than 38 % of total protein secreted was screened. the foreign protein expressed amount to 10mg / l by estimate
我們篩選到一株高表達的菌株gtr48 ，經過甲醇誘導之後trail基因表達量占總的酵母分泌蛋白的38以上，並且初步估計其表達量可以達到每升十幾毫克以上。 -
In the second method, the protein is secreted across the cytoplasmic membrane using the signal peptide method and then two periplasmic helper proteins facilitate the movement of the protein across the outer membrane
第二種方法是用信號肽方法分泌到細胞膜外，然後兩種周質幫助蛋白促進蛋白移出外膜。 -
The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
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