selective gene expression 中文意思是什麼

selective gene expression 解釋
選擇性基因表現
  • selective : adj. 1. 選擇的；挑選的；有選擇性的；淘汰的。2. 【無線電】選擇性的。adv. -ly
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  2. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  3. We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going

    構建了用於水稻中表達的載體pca - ced9 ，通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞，經潮黴素（ hym ）篩選，以期望獲得抗性植株，目前該工作仍在進行中。
  4. First, to enrich the selective markers for u32, two resistance genes emr and cat, one report gene amy were introduced into u32 to test their expression

    首先對適用於u32的選擇性標記進行了篩選，結果表明來自puc19e的紅黴素抗性基因和來自pulam2的澱粉酶基因可以作為u32的選擇性標記。
  5. Bar gene also is one of widely used selective marker gene. the cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology

    而且bar基因也是迄今為止應用最為廣泛的一個抗除草劑選擇標記基因，克隆出bar基因對植物的遺傳轉化，基因表達和生理學研究都有重要的作用。
  6. In the second part of the thesis, we described that a tobacco chloroplast expression vector, ptrvp1, containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method

    論文第二部分主要敘述了煙草葉綠體表達載體ptrvp1的構建，並通過基因槍方法轉化煙草葉綠體基因組，獲得了3株具有壯觀黴素抗性的轉化再生植株。
  7. 2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region

    以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。
  8. First, a plant expression vector, pbbbt was constructed by inserting tsase into pbbb - etr to substitute the etr gene within the plant expression vector. then, it was introduced into the super - poison agrobacterium tumerfaciens eha105 through the " triparental mating " method. twenty regenerated tobacco plantlets were obtained with selection medium under the ppt selective pressure

    首先將tsase基因取代植物表達載體pbbb - etr的etr基因構建植物表達載體pbbbt ，然後利用「三親交配」法導入超毒性農桿菌菌株eha105中，接著通過農桿菌介導法遺傳轉化煙草，經過連續的ppt抗性篩選，獲得20株抗性植株。
分享友人
分享到 Line

分享到臉書