sephadex g- 中文意思是什麼

sephadex g- 解釋

  • sephadex : 降膽葡胺
  • g : 1. generator. 2. German. 3. Germany. 4. giga 十億。5. Gossypium 棉屬。6. grid. 7. Gulf. 8. 【軍事】 gun.
  1. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  2. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
  3. 3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg

    -葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ，並對其專一，不能水解棉花和羧甲基纖維素鈉；分子量為117 . 5kda ，加dtt後分子量不變；該組分最適ph和溫度分別為4 . 5和70 ，在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ，最大反應速度vm為0 . 088mg葡萄糖（ ml ? min ） ；與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現，該組分是一個新的-葡萄糖苷酶。
  4. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  5. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  6. In order to attain bioactive glycoprotein, glycoprotein of the leaves of camellia sinensis, was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography, by cona - sepharose 4b affinity chromatography

    為了純化天然糖蛋白，進行了茶樹葉糖蛋白的cona - sepharose4b親和層析，從sephadexg - 100凝膠過濾收集的粗糖蛋白中，分離茶樹葉糖蛋白。
  7. After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform 、 ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back

    用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。
  8. An antifungal protein, named as b16, was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate, desalt and gel filtration on sephadex g - 75, which with molecular weight at about 30 - 40 kd on the basis of sds - page

    菌株041381發酵上清液經70飽和度硫酸銨沉澱，透析脫鹽， sephadexg - 75凝膠過濾層柱，得到活性物質b16 。以sds - page膠為基礎進行電泳分析， b16的分子量為30 - 40kd 。
  9. The antimicrobial secretion could be divided into three sections, every section had antimicrobial activity. the first could be extracted by petroleum ether, the second could be extracted by ethyl alcohol, the third could dissove in water and could be separated by sephadex g - 50, g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256, . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi, they could inhibit sixteen kinds of plant pathogenic germs, eight kinds of animal germs and eight kinds of plant pathogenic fungi

    粗提物經石油醚萃取可得第一個活性部分，剩餘部分經無水乙醇萃取可得第二個活性部分，剩餘物質再經凝膠sephadexg - 50後有兩個峰，第二峰有活性，再經凝膠sephadexg - 200後有三峰，第二峰有活性，將其經過陽離子交換柱cm - 1後有兩峰，此兩峰均有抑菌活性。
  10. Mt - were prepared from the rabbit liver induced by zinc after isolation and purification. they were demetalled in acidic condition, then applied sephadex g - 25 to gain apomts free of metals

    從鋅誘導的家兔肝臟中分離純化得到兩種亞型的金屬硫蛋白mts ： zn
  11. 3. the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100. the specific activity of the purified enzyme was increased by 37. 9 folds over crude extract, and the recovery yield was 8. 9 %

    以粗酶液為起始，經硫酸銨分級沉澱、 sephadexg - 100凝膠過濾后，分離純化了該酶，純化倍數約為37 . 9 ，酶活力回收率8 . 9 。
  12. The crude cellulases from liquid fermentation of b - 6 and ass. 3711 were isolated and purified by ( nh4 ) so4 precipitation, sephadex g - 100 and deae - sepharose cl 6b column chromatography. the cmcase components were purified and some of their physical and chemical properties were studied

    本文將液體發酵的酶液經硫酸銨分級沉澱、柱層析后得電泳純cmcase組分，並對as3 . 3711和b - 6來源的cmcase酶解動力學和理化性質作了比較研究。
  13. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
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