sequence specific primers 中文意思是什麼

sequence specific primers 解釋
序列特異性引物
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  • specific : adj 1 特殊的；特有的；特定的，專門的。2 明確的，具體的。3 【生物學】種的；【細菌】專性的。4 【醫...
  1. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  2. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  3. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  4. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  5. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  6. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  7. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  8. But the vvidbv strain gx8 / 99 that had been identified by gui only have saci side, and no sspl, the reason need farther research. according to the specific different sequence between cibdv and vvibdv in vvp2 gene. we also designed two sets of primers ( cibda + cibds and vvibda and vvibds )

    另外，針對不同強弱毒株的1 v的pz高變區序列的差異：設計合成了vvibdv的型特異性引物（ ibda vvibds人建立型特異的rt pcr ，只需一次反應即可區分cibdv和vvibdv 。
  9. The main difficulty in development of microsatellite ( or simple sequence repeats, ssr ) is to screen the specific sequences flanking ssr, a method to screen microsatellite based on sampl was tested in the chinese shrimp. randomly selective bands ( s1k s13 > s14 > s22 > s24 ) were cloned and sequenced, 61bp, i57bp 147bp, 152bp, 298bp sequences were obtained respectively. no ssr were found inslk s s s22 apart from the sampl primers sequence, on the other hand, a ssr sequence of ( ga ) 39 were found in s24, which comprises aflp primers only

    微衛星的發展主要受限於微衛星兩端旁側特異引物的篩選，本研究在中國對蝦中初步嘗試了基於sampl技術的微衛星標記開發，隨機選取了5個s枷pl條帶（ 511 、 513 、 514 、 522 、 524 ）進行了克隆測序，大小分別為161bp 、 157bp 、 147bp 、 152bp 、 298bp ，但在511 、 513 、 514 、 522四個片段中未找到引物序列外的微衛星序列重復; 524兩端引物均為傳統的aflp引物，在此測序片段中有一（ ga ） 39重復序列。
  10. Two specific primers were designed and synthesized based on the conservative sequence contrasted to drosophila and other species mt gene by computer retrieving, which were named primerl and primer2, the sequence of primer1 was : 5 " caa gtg aat cat ctc agt gc3 ", and that of primer2was : 5 " tgt aga gag aca aga tgc ag 3 ". rt - pcr technique was adoped to synthesizing the first - band of cdna, the second - band of cdna and expanding dna directly only by one step

    根據已發表的果蠅以及其它生物的mt基因的保守序列，通過計算機同源檢索比較，設計兩個擴增引物，命名為引物1和引物2 ，引物1的序列是： 5 』 caagtgaatcatctcagtgc3 』 ，引物2的序列是： 5 』 tgtagagagacaagatgcag3 』 。
  11. According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat, we designed primers and got the structure gene successfully. by 3 ' - race method combined with nested pcr, the 3 ' - end nuclear acid sequence was also obtained ; in additon, for the 5 ' - end sequence, we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer, and till now, partial 5 ' - end sequence has been amplified as well

    本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms （質譜法）測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物，擴增克隆了該過敏蛋白結構基因的編碼序列；根據測得的序列設計特異性引物，並利用3 』 - race方法結合巢式pcr擴增得到基因的3 』末端；依據同源性比較的結果選用一段保守序列為5 』引物，並根據結構基因內部序列設計3 』特異性引物，進一步獲得了該基因5 』端的部分序列。
  12. The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively

    Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上，結合已發表的文章及基因序列，針對senv - d和senv - h基因組設計特異性引物，利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株（ senv - d - bj1 ）的全部編碼區基因序列，還得到了一個senv - d亞型分離株（ senv - d - bj2 ）的大部分編碼區基因序列（包括orf1和orf2 ） ；從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株（ senv - h12 - 1和senv - h12 - 2 ）的全部編碼區基因序列。
  13. In this research, total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ). based on reported sequence of hsa cdna, specific primers were designed to amplify its encoding region by rt - pcr. at the same time, kozak sequence was added to the translation site, to strengthen initiation of translation

    本研究通過改進的異硫氰酸胍-酸性酚-氯仿一步法從人工流產胎兒肝臟中提取總rna ，然後根據報道的序列設計特異性引物，同時在上游引物中引入了kozak序列，經rt - pcr克隆到hsacdna編碼區序列，長度為1758bp 。
  14. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  15. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  16. Extract the total rna from human tonsil and amplify the h d - 3 specific cdna sequence using rt - pcr with two special primers based on h d - 3 amino acid sequence in genbank. then the amplified product was analyzed on a 2 % agarose gel

    自人扁桃體組織提取細胞總rna后，根據genbank中編碼h d - 3成熟肽45個氨基酸的cdna序列，設計特異引物進行rt - pcr 。
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