sequence-specific 中文意思是什麼
sequence-specific
解釋
序列特異的-
It also carries a specific nucleotide sequence, the anticodon.
它還帶有特定的核苷酸序列即反密碼子。 -
In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank
本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。 -
Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。 -
To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative
目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。 -
We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression
對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。 -
Described here is the cloning and characterization of a new trichome - specific - promoter in arabidopsis. by ddrt ( differential display reverse transcription ) - pcr and reverse northern, a 280bp sequence is acquired from the epidermal in viciafaba, which is specially expressed in the epidermal. then using race, we abtain a 3. 0 kb gene segment including the 3 ' end full sequence and the 5 ' end partial sequence of the 280bp segment
首先，我們利用差異顯示技術和反northern技術，以蠶豆葉肉細胞為對照，從蠶豆葉片表皮細胞中克隆出長280bp的新的表皮特異表達基因片段，再通過race技術獲得此基因片段的3 』端全序列和5 』端部分序列。 -
There are two kinds of dynamic analysis : aggregated analysis based on the from multiple execution of the process model elements and case analysis using one instance of execution of a specific sequence of process elements
存在兩種動態分析：聚合分析（基於多個流程模型元素的執行過程）和實例分析（使用流程元素的特定序列的執行實例） 。 -
As a member of an important transcription factor family, ap - 2 a expresses in specific tissue and binds with specific dna sequence
Ap - 2作為一個重要的轉錄因子ap - 2家族的成員，具有組織表達特異性和dna結合特異性。 -
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method
Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。 -
The back - exchange process is actually complex and deserving of analysis in a sequence - specific manner
返交換實際上很復雜，需要進行序列特異性的分析。 -
However, the cloned promoter had 18 substitution at 18 sites, 22 deletions at 18 sites and 3 insertion at 3 sites between sites 0 - - - 1273 bp which was reported to control temporal - spatial specific expression, and 3 substitution at 3 sites, 6 deletions at 6 sites between - 1095 bp and - 1273 bp, the key functional sequence area, in comparing with known osg6b
但是與報道的osg6b比較，在決定時空特異性的0 - 1273bp功能區域內，有18處出現18堿基替換， 18處出現22堿基缺失， 3處出現3堿基插入；在核心功能序列區域（ - 1095bp - 1273bp ）內，有3處出現3堿基替換， 6處出現6堿基缺失。 -
Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence
用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。 -
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。 -
The specific research methods include : the reliability and validity of the scale ( cronbach ’ s of the scale, split - half reliability, the reliability of each dimension, discriminant validity, convergent validity ) ; the factor analysis method to get the dimensions of internal service quality ; the independent - samples t - test and paired - samples t - test method to analyze every discrimination of internal service quality ; the comparison of means to evaluate the sequence of every dimension
具體的研究方法包括:對量表進行信度和效度分析，包括整個表的cronbach系數、分半信度、各維度的信度、區別效度和收斂效度的分析;使用因子分析的方法測量內部服務質量包含的維度;採用兩獨立樣本的t檢驗和兩配對樣本的t檢驗的統計方法對內部服務質量各差距進行分析;通過對樣本均值的比較，分別得出各維度在員工和管理者心目中的重要性排序。 -
This paper mainly focuses on the noise limiting by means of the direct sequence spread spectrum ( dsss ) and the analysis of the transmission performance of the plc and some digital modulation technology. the contents of the paper is as follows : 1 ) the technical feasibility is proved after simulating noise limiting principle of dsss by means of systemview, the simulation software ; 2 ) a kind of band pass filter ( bpf ) is realized according to the requirement of filter and the principle of butterworth approximation, which satisfies the index of performance of dsss. 3 ) the low voltage plc system includes the sc1128, the specific modulation / demodulation ic, the bpf filter and other circuit components, furthermore, the control function of system is realized by means of the personal computer and the microcontroller
本課題在對低壓電力線的傳輸特性和數字調制技術進行分析的基礎上，將通信理論中的直接序列擴頻技術（ dsss ）用於解決低壓電力線通信的干擾問題，主要研究內容如下： （ 1 ）用通信模擬軟體systemview對dsss技術的通信和抗干擾原理進行模擬分析，分別對時域和頻域下採用dsss技術前後接收信號的頻譜進行分析，驗證dsss技術在本系統中的可行性； （ 2 ）由dsss技術對濾波系統的要求，根據濾波理論分析了巴特沃思型濾波器的逼近原理並設計了合適的濾波電路； （ 3 ）用調制解調晶元sc1128和自行設計的濾波器加之輔助外圍電路，構造出低壓電力線載波通信系統，並採用atmel公司的單片機設計了接收和發射電路的微控制器； （ 4 ）分別對採取抗干擾措施前後輸入和輸出信號進行對比實驗，並對結果進行分析，驗證了dsss技術對干擾信號的抑制作用。 -
But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products
細菌mrna多聚腺苷酸化的位點多種多樣，包括初級轉錄產物的3 』末端， 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內，其腺苷酸化相對無特異性、無選擇性。 -
The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot
4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。 -
Result : the decision tree consisted of multiple levels of branches and color blocks to present the output and the sequence of information gathered ( e. g., length of stay > disease classification > mode of departure from the hospital > triage > medical specific ) and reflected the degree to which the distribution of medical expenses were influenced
結果：決策樹以多層次之樹枝分佈及顏色區塊等視覺化方式呈現研究結果；其中資訊增益順序為（滯留時間疾病分類離院后動向檢傷分級科別） ，該資訊增益之順序也代表屬性影響醫療費用分佈之程度，意即滯留時間為決定急診病色醫療費用多寡之首要因素。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。 -
In this paper, a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied. in addition, we synthesized and analyzed a sequence - specific cleavage reagent, in which oligodeoxynucleotide was recognizing group. detailed work mentioned below were carried out : 1
本文在查閱大量文獻的基礎上，以1 ， 10 -鄰菲咯啉和1 ， 10 -鄰菲咯啉- 5 ， 6二酮為配體合成了一系列過渡金屬配合物，詳細研究了它們與pbr322dna的相互作用及其機理。
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