sequencing 中文意思是什麼

Cloning and sequencing of acc oxidase gene from sugarcane

氧化酶基因片段的克隆與序列分析

By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。

The chinese university sequencing data will be submitted electronically to the who working group on aetiology of sars

中大研究成的基因序列將以電郵發放予世界衛生組織的sars病理研究聯絡組。

The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。

The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。

Sequencing dna has come down in price by half annually, from $ 10 per base pair in 1990 to under a penny today

資訊科技通常不要一年，價格表現比和產能就會增加一倍。

Capsid proteins are involved in recognition and infection of the host and can be used to identify the virus by serological means or amino acid sequencing

衣殼蛋白與寄主的識別和感染有關，還可以通過血清學的方法或氨基酸序列來識別病毒。

Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。

Bioinformatics methods are used to analyze and manage expressed sequence tags ( ests ) which are generated by large - scale sequencing of a cdna library from cephalothorax of a wild, female, adult chinese shrimp ( fenneropenaeus chinensis )

本文運用生物信息學的方法，分析及管理由雌性成年中國對蝦頭胸部cdna文庫經部分測序產生的10446條ests 。

Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing

方法利用酶切及sscp技術檢測凝固酶基因序列改變情況，並測序進行確證。

Study on the water decanter in sbr sequencing batch reactor system

工藝用潷水器的研究

Conclusion the mtdna sequencing system using new primers was useful for forensic purpose. for difficult samples, dna typing with this system was especially available and practical, and has been validated in criminal cases

結論本課題所建立的線粒體dna序列分析體系是一種對法醫疑難檢材進行鑒別的有效、實用的方法，並且本方法已經在實際案件檢驗中得到驗證。

Cloning and sequencing of vp3 gene of avian encephalomyelitis virus

3基因的克隆與序列測定

Cloning and sequencing of flab gene of polar flagellin of vibrio parahaemolyticus

基因的克隆與序列分析

In the text, the forming process and the microbial ecology of the activated sludge floe in sbr ( sequencing batch reactor ) were observed and studied

摘要採用序列間歇式反應器（ sbr ） ，對啤酒廢水活性污泥凝絮體的形成過程及其微生態演替進行研究。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Cloning and sequencing analysis of sus scrofa hepatitis c virus core - binding protein 6 homologue gene

豬丙型肝炎病毒核心蛋白結合蛋白6同源基因的克隆化研究

In addition, the evident crossing - infection happened in the strains came from myrica, casuarina also could be found in the sequencial analysis. all these results we obtained from the sequencing and rflp analysis were partly accorded with what baker brought forward in 1987 ( the four host specific group, hsg ). however, they also indicated the limit of this hsg

5株供試菌株與其它已發表菌株的全序列比較結果可將所有菌株大致劃分為4個簇，楊梅菌株fmr61 、木麻黃菌株fcg07和木麻黃菌株fce42具有較高相似性與榿木菌株聚為第簇；而楊梅菌株fmr16和2215與來自胡頹子科和沙棘的菌株聚在一起歸為第簇。

Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon

對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。

One missense mutation was found in 48 hypertriglyceridemic patients and identified to be pro207 - leu by sequencing, pedigree analysis of this proband has been carried out and showed that the pro band ' s father is also a carrier of this mutation

徵得患者家屬的同意和支持，對其家族三代十人進行了完整的家系分析。家系分析顯示，先證者的突變來自其父親的遺傳。在外顯子6 、 7 、 8中未檢出基因突變。