signal peptide 中文意思是什麼

signal peptide 解釋
信號肽
  • signal : n 1 信號，暗號；信號器。2 動機，導火線 （for）。3 預兆，徵象。adj 1 暗號的，作信號用的。2 顯著的...
  • peptide : n. 【生物化學】肽；縮氨酸。
  1. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  2. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  3. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  4. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  5. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  6. To guide the cam - binding peptide into apoplast space, the signal peptide from brassica campetris pollen coat protein slr1 ? ps was employed. it has total 26 amino acid, and is a very classic form of signal peptide

    為了把cam結合小肽帶到質外體，本實驗選擇了野生蕓苔屬植物（ brassicacampetris ）花粉胞壁蛋白slr1 - bps的信號肽。
  7. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  8. The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2

    真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔， kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上，從而阻斷了蛋白質進入高爾基體和細胞質的過程，進而避免了外源蛋白質的異源糖基化修飾，延長了蛋白質在生物體內的半衰期。
  9. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  10. In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon

    序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。
  11. In this paper, signal peptide was added before the n - terminus of cema with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity

    本文通過在cema的n端添加信號肽，對其進行改造，以探索不降低抑菌作用的同時，降低其對植物細胞毒害作用的可能性。
  12. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  13. The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites, four conserved cysteine residues, two out - of - frame atg initiation codons in the 5 " untranslated region, and a signal peptide consisting of 66 amino acids. when it was compared with the published sequence of chil - 15 cdna, 7 mutant sites were found, and 5 amino acids were changed in predicted amino acids, which indicated that chil - 15 may be polymorphic

    結果顯示，本研究所用白來航雞il - 15cdna5 』非編碼區有兩個框外atg起始密碼子，開放閱讀框由564bp組成，編碼187個氨基酸，其中n末端信號肽含有66個氨基酸殘基，在第48 、 149和166位的天冬酰胺殘基上有三個潛在的n -糖基化位點。
  14. By means of pcr, the signal peptide dna sequence was deleted. for study of the limited factors in expression of pro - urokinase, the pro - uk gene was divided into three fragments, and they were expressed in

    最後用同樣的方法提高全長人尿激酶原cdna在大腸桿菌中的表達量，使其表達量達到全菌蛋白的5 % 。
  15. This paper studied the cloning signal peptide and promoter of grb - ast gene

    本論文對grb - ast基因的信號肽序列及其啟動子進行分離克隆的研究。
  16. Computer - assisted analysis revealed there was a signal peptide near the n - terminal of this protein

    軟體預測0rf132有一個信號肽序列（ signalpeptide ） ，最可能的切割位點在和r20之間。
  17. Sequence analysis shows that the n - terrninal 1 - 25 amino acid sequence is a predicted signal peptide

    序列分析也顯示蛋白n ?端具有信號肽序列特徵，表明ecbp21可能是一種分泌到細胞外的蛋白。
  18. Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco. the results of fluorescent detection showed gfp localization in the apoplast

    Elisa結果顯示，馬鈴薯蛋白酶抑制劑基因的信號肽序列使crylac基因在轉基因煙草中的表達量顯著提高。
  19. They inhibits the growth of fungi ( filamentous fungi especially ) while are non - toxic to plant cells. the main results were as follows : 1. obtaining of spcema ( signal peptide modified cema ) spcema ( 187bp ) was amplified with two long complementary primers ( p2 and p3 ) and two primers ( pl and p4 ) containing restriction enzyme recognition site

    帶信號肽cema基因的pcr合成以兩條部分重疊長鏈引物p _ 2和p _ 3延伸產物為模板， p _ 1和p _ 4為正、反引物進行pcr擴增，獲得了改造的抗菌肽基因spcema （ 187bp ） 。
  20. The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs. the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs. the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide, two krab domains and 14 c - terminal c2h2 zinc finger motifs

    Znf325長2697個堿基，含有一個長為1389個堿基的開放閱讀框，編碼一個長為462個氨基酸的蛋白質，它包含15個c2h2型鋅指結構； znf359長3270個堿基，含有一個長為1932個堿基的開放閱讀框，編碼一個長為643個氨基酸的蛋白質，它包含一個n -端krab結構域和16個c -端c2h2型鋅指結構； zfp28長4104個堿基，含有一個長為2076個堿基的開放閱讀框，編碼一個長為868個氨基酸的蛋白質，它包含一個n -端信號肽，兩個krab結構域和14個c -端c2h2型鋅指結構。
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