single fertilization 中文意思是什麼
single fertilization
解釋
單受精- single : adj 1 僅只一個的，單獨的；單式的；【植物；植物學】（花等）單瓣的；【無線電】單工的，單次的。2 獨...
- fertilization : n. 1. 肥沃化，施肥（法）；豐饒化。2. 【生物學】受孕[受精]（作用，現象）。
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In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。 -
Until recently the fertilization practice has been for a single crop, even though the sequential cropping system has been followed
即使已採用了連作種植制度，但是直到最近還是只對一種作物進行施肥。
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