soluble protein method 中文意思是什麼
soluble protein method
解釋
可溶性蛋白質度測定蛋白質變性-
Highly soluble protein - rich soyabean drink with modern scientific method, it is made from the finest soyabean, maltose and white granulated sugar, the product has rich vegetable proteins
產品特點冰泉牌速溶豆漿晶是以優質黃豆白砂糖為主要原料,採用現代科學技術精製成沖飲方便的速溶固體飲料。 -
After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。 -
In the second part, polyphasic taxonomy methods such as morphological method, physiological and biochemical method, dna g + cmol %, soluble protein electrophoresis and 18s rdna sequence analysis were used for systematics of all strains isolated and purified with the method above
本論文第二部分採用形態學、生理生化特徵、 dnag + cmol 、可溶性蛋白電泳及18srdna序列分析等分類技術對所分離的16株甲真菌進行了系統的分類研究,從而初步確定了16株甲真菌的分類地位。
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