stable cells 中文意思是什麼
stable cells
解釋
穩定細胞-
Stable suppression of afp gel expression by rnai in smmc - 7721 cells
表達質粒穩定轉染肝癌細胞克隆的構建 -
At the surface of the pollen grains there are three types of aperturates, including three porates type, three colpates type and three porate - colpates type ; and four types of sculpture, namely, reticulate, striate, striate - reticulate and verrucate. the upper epidermal cells of the leaves of 12 species plants are detected with hpias - 1000 image analytic system through the treatment of binarization, and the experimental methods are proved stable. the results indicated that the waviness of the anticlinal walls ( sfc ) and the ratio of the feret ' s diameter ( slf ) of the epidermal cells of the middle lamina in the third node of leaves starting from the lowerest part of the stem have a relative constant range and could distinguish from each other. oieanolic acid, l, 5, 8 - trihydroxy - 3 - methoxyxanthone and swertiamarin are separately detected on the tlc, and the different chromatogram of various plants can be considered as characters of identification. the contents of oleanolic acid in 12 species of plants are determinated by hplc, but among the different plants and botanical organs their contents are different, and the highest content in flowers
本文對川鄂產獐牙菜屬藥用植物資源進行了野外調查、標本採集和鑒定，對12種乾燥藥材的性狀進行了描述，提供了可以鑒別的特徵。通過掃描電子顯微鏡觀察的12種本屬藥用植物的花粉粒均為單粒花粉，萌發孔有3孔型， 3溝型和3孔溝型三種類型；表面紋飾包括網狀紋，條狀?網狀紋，條狀紋和瘤狀紋四種類型。用hpias ? 1000高清晰度彩色病理圖文分析系統對葉片上表皮細胞作圖像分析，並進行方法學研究，結果表明，同種植物莖上第3節葉片中段主脈和第1側脈之間葉上表皮細胞垂周壁彎曲程度sfc值和細胞縱、橫向直徑的比值slf值，種間有顯著差異，每種都有相對恆定范圍值。 -
( 3 ) firstly detectes the waviness of the anticlinal walls ( sfc ) and the ratio of the feret ' s diameter ( slc ) of the epidermal cells in the leaves of 11 species, and studies the methodology, then give out two conclusion : a. epidermal cells in the place between middle and first side vein of the lamina on the third kont starting from ground have the most stable microscopic characters
（ 3 ）首次採用計算機圖像分析「 hpias ? 1000高清晰度彩色病理圖文分析系統」測定了獐牙菜屬11種藥用植物葉上表皮細胞垂周壁彎曲程度（ sfc ）和細胞縱橫直徑比（ slf ） ，並進行了方法學研究，得到以下結論：莖上第3節葉片中段主脈與第一側脈之間上表皮細胞形態最穩定。 -
Study on construction of hepatocyte growth factor expression vector system and stable expression in rat bone marrow stem cells with
基因的逆轉錄表達載體及體外感染骨髓間充質幹細胞的研究 -
In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。 -
Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody. our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4. after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4. our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我們構建的rhpn原核高效表達系統經m page及凝膠密度掃描分析結果表明， rhpf4表達量占菌體總蛋白量的25 30 ，較原表達克隆pt7 7 rhpf4提高了近80倍，經快速高效的包涵體分高純化工藝和復性工藝， rhpf4具有野生蛋白活性。 -
The results showed that es cells derived from kunming albino mouse remained undifferentiated state when they were cultured in vitro for a long time, and karyotypies were stable, but the ability of germ - transmission have not been verified
結果顯示，用昆明白小鼠囊胚所建立的胚胎幹細胞在體外長期培養時仍能保持未分化的狀態，核型穩定，但還未證明其具有生殖系嵌合的能力。 -
The apoptosis in the passage cells was detected by using flow cytometry. it was fmded that the apoptosis of passage 12 cells was not obvious. cells were subcultured to sixteenth passage. it proved that the subculturing of porcine ear skin fibroblasts was stable by using this kind of method combining trypsin cold treatment with trypsin heat treatment
在進行傳代培養時，利用了流式細胞儀檢測了培養細胞的凋亡情況，發現傳至第12代的細胞仍無明顯凋亡現象，細胞已傳至16代以上，說明應用此方法可穩定地進行豬耳皮膚成纖維細胞的傳代培養。 -
In vitro model system, they found that stable - transfected cell lines which are over - express wnt - 1 or n - cadherin gene could differentiate into neuron - like cells without ra induction
目前從事的主要研究工作有：小鼠巢蛋白基因表達調控的分子機制；胚胎性癌細胞 -
The fusion gene ofgfp : mtn ( mtn is binding domain of microfilament binding protein talin, which can show the microfilament in living cell ) was transferred to arabidopsis thaliana. stable expression of gfp - mtn can visualize the actin cytoskeleton in different types in living cells without affecting cell morphology and function
將gfp : mtn （ mtn是微絲結合蛋白talin的微絲結合域，可以顯示活體細胞中微絲的結構）導入擬南芥后，發現表達的融合蛋白能夠標記微絲骨架，同時又不影響植物細胞的正常形態和功能。 -
This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。 -
Our study establish a stable base for further reseach of hng and human insulin mutant and provide a theoretics gist for expression of low molecular weight proteins in prokaryotic cells
為用融合表達方式表達小分子量多膚及規模化制備小分子蛋自奠定了初步的實驗和理論基礎。 -
Poly ( 3, 4 - ethylenedioxythiophene ) is one of the most environmentally stable and promising inherently conducting polymers. it ' s applications in organic optoelectronics, especially in organic electroluminescence and solar cells are reviewed
摘要簡要綜述了高性能導電高分子聚（ 3 ， 4 -二氧乙基噻吩）在有機光電子領域，特別是在有機電致發光和有機太陽能電池中的應用。 -
Even then, they were able to establish only two stable cell lines from some 91 initial cells
即使這樣，他們從91個最初的胚胎細胞進培養出2個穩定的胚胎幹細胞細胞群。
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