stage purification 中文意思是什麼

stage purification 解釋
多級凈化
  • stage : n 1 講臺；舞臺；戲院，劇場；〈the stage〉戲劇，戲劇藝術；戲劇文學；〈the stage〉戲劇業；劇壇。2 ...
  • purification : n. 1. 清洗，洗凈；凈化（作用）。2. 提純，精製。3. 【宗教】滌罪；潔身；齋戒；潔禮，祓；【天主教】潔杯式。
  1. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  2. Zla serial can be utilized in purification of various types of insulation oils, especially deep purification treatment of high - stage new oil, imported oil and super - voltage transformer oil

    廣泛適用於各類電力設備的絕緣油凈化處理。特別是對高等級的新油進口油和超高壓變壓器油的深度凈化處理。
  3. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  4. The structures of phenylethanoid glycosides in plant extract of cistanches deserticola were successfully analyzed and identified by electrospray ionization multi - stage mass spectrometry ( esi - ms ( superscript n ) ) in negative mode, some interesting neutral losses were found, which helped us to confirm the structures of compounds without tedious separation and purification procedures, then the fragmentation mechanisms were explored

    摘要採用電噴霧多級串聯質譜技術，對肉蓯蓉藥材粗提物中的苯乙醇苷類化合物的特徵質譜行為進行了系統研究，根據一些特徵的中性碎片丟失，提出了該類化合物的質譜裂解規律。
  5. This subject uses the genetic engineering technology and the newest modern biotechnology to study the genetic engineering lower reaches stage, in order to research and establish the optimum, high density fermentation technology pattern and high efficient isolation and purification model of recombinant proteins from inclusion bodies

    本課題利用基因工程技術，重點從基因工程下游階段著手研究，利用最新現代生物技術研究並建立經優化的高密度發酵工藝模式及高效分離純化包涵體重組蛋白模式。一
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