streptavidin-biotin complex 中文意思是什麼
streptavidin-biotin complex
解釋
抗生蛋白鏈菌素生物素復合體- streptavidin : 抗生蛋白鏈菌素,抗生物素蛋白鏈菌素
- biotin : n. 【生物化學】生物素，促生素，酵母生長素，維生素H。
- complex : adj 1 復雜的，錯綜的。2 合成的，綜合的；【化學】絡合的。3 【語法】復合的；含有從屬子句的。n 1 復...
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Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex
將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大，實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后，傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應，從而使傳感片表面特異健合的物質質量顯著增加，大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后，可檢測0 . 00510g ml濃度區間內的higg 。 -
Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。
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