subcloning 中文意思是什麼

subcloning 解釋
亞克隆
  1. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  2. The bvdv nadl strain is cytopathogenic. searching fromgenbank several cp strains " sequence of e2 gene about deer - n21, sh9, newyork - i and so on were found. according to the homologous sequence they were designed and synthesised a pair of primers by the biology software primer 5 and added bal i and nco i site to the 5 " end which can be used for the application of e2 gene and subcloning

    從genbank中搜尋出deer - n _ （ 21 ） 、 sh9 、 newyork -等cp型毒株的e _ 2基因序列，根據其同源性用生物學軟體primer5設計引物，並在引物的兩端加入bal和nco兩個酶切位點，酶切位點的存在易於對e _ 2基因進行克隆操作。
  3. Plant expression vector pespcema was constructed after subcloning in puc121 and other vectors

    經puc121等中間載體的亞克隆，構建了pespcema植物表達載體。
  4. As heterologous probe and subsequently show to code for desired enzymatic activity. after a serial of subcloning coupled with southern hybridization and enzymatic activity assay, the functional s. griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2. 3kb ecori - sall fragment

    對phz1140和phz1141進行bamh及bgl的酶譜分析及與choa探針的雜交，將膽同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。
  5. Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe

    對片段進行克隆、亞克隆之後測序分析，發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性；並且三次獨立的pcr均得到相同的片段，所克隆的基因序列與choe相比，連續缺失三個堿基（ ttc ） ，即相應的氨基酸序列缺失一個氨基酸（ phe ） ，因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體，命名為choew 。
  6. One solobp ecor i fragment containing phospholipase gene was isolated. further sequence analysis and subcloning revealed a 963bp phla. gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd

    通過測序及亞克隆分析，發現一個磷酯酶的基因phla ，長度為963bp ，預測編碼一個由320個氨基酸組成，分子量為33kd的磷酯酶phl 。
  7. With a series of hybridization and subcloning, the two deletion end - points of this deleted region and deletion junction were localized precisely and cloned

    通過southern雜交和亞克隆，精確定位和克隆了這段缺失區域的兩個端點及缺失界點。
  8. The screening of supernatants was carried out with using indirect elisa. after subcloning by limiting dilution and selection. five clones of cells with high od value were acquired and named mvi

    過陽性雜交瘤細胞的篩選和有限稀釋法的克隆化步驟，獲得了5株od值高的單克隆細胞株，編號為mv1 ， mv2 ， mv3 ， mv4 ， mv5 。
  9. Subcloning of 32kda proteins gene of schistosoma japonicum in the eukaryocyte expression vector

    蛋白質基因在真核表達載體中的亞克隆
  10. The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase

    以pgem - 3zf和pbluescript為載體，利用限制性內切酶hind和pst構建這兩個克隆的亞克隆，從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ，插入片段各為2 . 4kb和3 . 2kb ，對這兩個亞克隆進行序列分析，發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列長為1323bp ，推導的epsp合成酶由441個氨基酸組成，兩個亞克隆的草甘膦耐受濃度最大可達150mm 。
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