supplemented medium 中文意思是什麼

supplemented medium 解釋
補給性培養基
  • medium : n （pl dia ）1 媒介物；傳導體；媒質，基質，介質，介體；中間物；環境、生活情形。2 手段，方法；媒介...
  1. High frequency of bud induction was found on ms medium supplemented with 6 - ba the optimum medium for adventitious bud induction was ms + l. 0mg / 1 naa + 0. 5mg / l 6 - ba, and ms + 1. 5mg / l naa + 0. 5mg / l iaa for bud rooting

    門）誘導毛狀根體細胞胚再生植株。通過正交實驗篩選出了毛狀根體細胞胚誘導的最佳培養基為： ms 05mg lnaa 0 sing kt 30gil蔗糖。
  2. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  3. In addition, we designed a test : the midgut was removed from spodoptera exigua larvae and was cut longitudinally into halves, the midgut epithelia were cleaned and digested with dispase ii, the pelleted cells in grace medium with 7 % fbs supplemented with 100units / ml penicillin, 100ug / ml streptomycin were cooled and then suspended in r18 - labelled virus

    實驗結果表明，在幼蟲的病毒接種物中添加合適濃度的熒光增白劑（ 0 . 01g . ml一』 ）不會改變病毒在幼蟲中腸組織中的感染部位，卻可以提高幼蟲對病毒的敏感性，增加了病毒毒力。
  4. Methods : hepatocytes obtained from mice with in situ portal vein collagenase perfusion were inoculated on two types of medium, which one was 10 % pcs rpmi 1640 medium ( common medium ), the other was rpmi 1640 medium supplemented with 5 u g / ml tranferrin, 5 u g / ml insulin, 10nm nicotinamide, 5mm - me, 40 u g / ml hgf ( special medium )

    一種是在rpmi1640培養液中加入了511iml轉鐵蛋白、 5pg ml牛胰島素、 10nm煙酚胺、 smm卜琉基乙醇， 4011g ml促肝細胞生長因子，簡稱為肝細胞培養液。
  5. Adventitious shoots could be rooted and formed regenerated plants on 1 / 2 ms medium supplemented with naa0. 4mg / l. hairy roots could be induced from the basal surface of the explants 8 days after inoculation of a. ficoidea cv. " ruliginosa " leaf explants with a. rhizogenes atcc 15834

    紅龍草葉片外植體被發根農桿菌atcc15834感染8d后，從形態學下端葉脈處陸續分化出乳白色的不定根， 21d后，葉片外植體的生根率高達92 . 5 。
  6. Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba, cppu, tdz or kt ) alone or in combination with naa or iba, and the adventitious buds regeneration rate was evaluated.

    以榨菜子葉、真葉為外植體進行離體培養，用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子葉、真葉再生不定芽試驗。
  7. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  8. After co - cultivation, all the explants were washed in liquid mso medium containing 500mg / l cef for 2 hours. washed with sterile water for 5 times and patted dry on the filter paper again. subsequently, the explants were transferred to ms1 medium supplemented with 200mg / l cef for 3 - 6 days delay selection and then transferred to ms1 medium supplemented with 200mg / l cef and 50mg / l km for selection culture

    對普通pcr檢測兩種結果均為陽性的轉化株系，又同時以355和nptll為目的片段進行2xmpcr檢測，結果得到tg一rch卜n二l一20 （表示檢測結果為陽性的轉基因矮牽牛rch卜有加個株系） 、 te一rch ，一n = z一10和to一wch ， + n = l一10 。
  9. Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2. 0mg / l 6 - ba and l. omg / l naa. the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods

    通過將不同濃度的秋水仙堿加入培養基和不同濃度的秋水仙堿溶液浸泡兩種方法處理愈傷組織，從而篩選出最佳的誘導方法和最佳的秋水仙堿濃度和處理時間。
  10. Pbmc were cultured in complete medium and supplemented with 1000u / ml rhgm - csf and 500u / ml rhil - 4, modc were collected on day 9. tumor cells were cultued with 10 % complete medium

    腫瘤細胞的培養將mgc803 、 ls174t 、 ec109細胞分別培養于含10胎牛血清青霉素100n分ml ，鏈黴素100n分ml的rpmi
  11. The hypocotyl segments of cabbage were used as the explants and cultured on a ms medium supplemented with plant hormone, which was established a cabbage regenerated system. the differentiation rate of adventitious buds was above 80 %. in this study, ubic gene was transformed to cabbage through agrobacterium mediated system

    同時，採用甘藍無菌苗的下胚軸為外植體，以ms為基本培養基，分別用直接分化再生系統和愈傷再生系統誘導不定芽的分化，建立了高效穩定的再生系統，不定芽分化頻率達80以上。
  12. 2. the strain can grow on ycg medium supplemented with amp, up to the concentration of 400ug / ml, whilst the original one cannot grow on the same medium even at 50ug / ml of amp

    而且在ycg培養基中， amp濃度高達400ug ml時仍有菌落生長。同樣條件下， ss - ori菌在amp濃度為50ug ml時就不生長。
  13. The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates

    加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。
  14. After 15h of maturation. 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended. secondly, strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium

    第一次減數分裂期小鼠卵母細胞經化學去核后，去除透明帶，並胎兒成纖維細胞粘合、電融合併srcl2激活，培養于微孔中。
  15. When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin, the ratio of germinated to non - germinated seeds is 3 : 1, which is consisted with mendel ' s first law. southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants

    轉基因植株的l代種了在潮黴素培養基上的萌發數與未萌發數的比值為3 ： l ，符合單基因的盂德爾分離規律。
  16. The culture was shake - cultured at 30 for 40 hours in liquid medium ympd supplemented with 0. 5 % glycine

    獲得了灰色鏈黴菌原生質體形成和再生的最優條件，即用ympd培養基，加0
  17. The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells

    研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。
  18. 2 established the regeneration system with mature embryos of rice ( 1 ) the optional inducing medium of callus is ms basal medium supplemented 3mg / l 2, 4 - d, 30g / lsucrose and 8g / l agar. ( 2 ) the rate of callus induction varied with the genotypes. dongnong v - 10 has the highest inducing rate among 4 tested genotypes, up to 96. 1 % ; the second is song 93 - 8 and dongnong 419, and the average inducing rate renched 88. 9 % and 88. 3 % respectively

    （ 2 ）不同基因型愈傷組織誘導率有差異，供試的4個基因型中，東農v10誘導率最高，平均誘導率達到96 . 1 ；其次是松93 - 8和東農419 ，平均誘導串分別為88 . 9和88 . 3 ；富士光誘導率最低，平均誘導率為78 . 9 。
  19. The result indicated : the basic medium supplemented 15 % ~ 20 % nbs or fcs was much easier to culture mef and es cells in vitro

    血清濃度的適度增加可提高細胞的活力和生長速度，並可延緩細胞壽命。
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