t rna 中文意思是什麼
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Secondly, the trends of dna and rna in the oocytes and follicle cells were displayed by histochemical method during oogenesis, which indicated that rna were synthesized at stage of the vitellogenic oocytes. but the quantity of dna was n ' t change
並根據這八個階段對卵子發生進行了組織化學的初步研究，結果表明，在卵子發生過程中，卵母細胞遺傳物質dna的總量相對保持不變，保證了遺傳信息的。 -
A new method for intrinsic terminator prediction based on rnall, an rna local secondary structure prediction algorithm developed recently, and two u - tail score schemas are developed. by optimizing three parameters thermodynamic energy of rna hairpin structure, u - tail t weight, and u - tail hybridization energy, the method can recognize 92. 25 of known terminators while rejecting 98. 48 of predicted rna local secondary structures in coding regions negative control as false intrinsic terminators in e. coli. this method was applied to scan the genome of synechococcus sp
在過去二十年中，不少研究者已開始研究如何用計算方法來預測轉錄終止信號，如brendel和trifonov的雙核苷酸分佈矩陣法dinucleotide distribution matrix carafa等的統計方法transterm和rnamotif法等，這些方法都從不同方面考慮了rna二級結構和u -尾部的特徵，而gester的預測模型則設定rna二級發夾結構是不依賴終止子的唯一因素。 -
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
In this research we have selected 4 pigs which exhibiting obvious muscular hypertrophy in the hindquarters, and 4 pigs with normal phenotype. we extracted total rna from their longissum tissue. part of the clpg cdna have been obtained by rt - pcr, but we did n ' t find the a g substitution as indentified in sheep
在本實驗中，我們選取了4頭在臀部具有明顯肌肉肥大表型的豬和4頭正常豬，並從它們的背最長肌中提取了總rna ，通過rt - pcr的方法得到了部分clpg1的序列，但是我們並沒有得到a g的單堿基突變。 -
Our results showed that, in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells, actin bound to brgl, nfl / ctf and rna polymerase ii during gene transcription. whereas, in unactivated mouse spleen t lymthocytes, no binding could be found
結果表明，在活化的小鼠脾t淋巴細胞和自主增殖的jurkat細胞中，肌動蛋白可以與mf復合物、轉錄因於nfi ctf和rna聚合酶11結合。 -
[ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t
L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。 -
4 ) all groups did n ' t show out the significant time - effect and close - effect relationships between pollutants and ( he soluble protein. but with the prolonging of exposure, the effect of all pollutants on rna and dna contents almost is that the content first increases and then decreases, and the larger the concentrations of the same pollutant, the earlier the decrease turns up
隨人物門問的延，所何污染物對廠n八、 1 ） n八念品的2即對從小卜斯見為先1 ： i門廠價的時間效應人系，九染物濃度越人；腳；川廠見下階門。 -
Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。 -
The total rna was extracted from human normal kidney tissue and the cdna fragment of hnadcs was produced by rt - pcr, then was purified and inserted into pgem - t vector. after dna sequenc - ing, pgem - hnadcs was digested with ecor i and sal i, and ? the dma fragment was subcloned into the ecor i and sal i sites of the fusion expression vector ( pgex - 5x - 1 )
從正常人腎組織中提取總rna ， rtpcr擴增hnadc3抗原表位區cdna片段，產物克隆至pgem嚇載體，測序正確后，將hnadc3抗原表位區dna片段再次亞克隆至pgex融合表達載體，構建重組質粒pgex十nadc3 。 -
Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。 -
After rt - pcr of total rna isolated from the ovaries of lagurus lagurus, the lzp3 cdna was cloned into pucm - t and further identified with sequence analysis
將擴增產物連接到pmd18 - t載體上進行測序，序列分析表明已克隆出lzp3全長cdna 。 -
2, immuno - co - precipitation showed that in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells, brg1, nf1 / ctf and rna polymerase ii were closely bound together, and this was not found in un - activated cells
2 、免疫共沉澱實驗證明，在活化的小鼠脾t淋巴細胞和自主增殖的jurkat細胞中， brg1 、 nf1 ctf和rna聚合酶是緊密結合在一起的，在沒有活化的小鼠脾t淋巴細胞中這三種蛋白質沒有免疫共沉澱現象。 -
In this dissertation, jurkat cells, hela cells and mouse spleen t lymphocytes were chosen as experimental materials to answer the question. with the aid of various techniques such as elisa, immuno - co - precipitation, indirect immunofluoresent co - localization, double - labeling immunoelectron microscopy and so on, the relationships of baf complex with nf1 / ctf and rna polymerase ii were careful observed and analyzed
本文以jurkat細胞、 hela細胞和小鼠脾t淋巴細胞為研究材料，通過酶聯免疫吸附實驗（ elisa ） 、免疫共沉澱、免疫熒光共定位和免疫電鏡雙標記等實驗，觀察和分析了baf復合物與轉錄因子nf1 ctf和rna聚合酶在基因轉錄活動中的相互聯系。
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