tannase 中文意思是什麼
tannase
解釋
丹寧分解酶-
Purification and some properties of tannase from aspergillus niger
黑麴黴單寧酶的純化及部分性質研究 -
Tannin acyl hydrolase ( tannase ) is an enzyme that hydrolyzes the ester bonds of tannic acid to produce gallic acid and some other compounds. tannase is widly used in beverage, food, leather and chemical industry
單寧酶（ tannase ）是一種水解酶，能夠水解沒食子酸單寧中的酯鍵和縮酚酸鍵，生成沒食子酸和其它化合物。 -
In china, tannase is obtained normally by the fermentation of natural aspergillus strains. the cell wall structure of this microorganism is very complex and the tannase was fixed tightly between the cell wall and the plasma membrane, which made the extraction and purification of this enzyme very difficult
目前國內單寧酶的生產主要是依靠麴黴的發酵來進行，由於麴黴的細胞壁結構比較復雜，而單寧酶是一種胞內酶，牢固地結合於細胞的細胞壁與細胞膜之間，這使得發酵后的分離純化工作非常繁瑣。 -
The activity of tannase was not detected
Sds - page檢測也未發現明顯的特徵帶。 -
The three recombinant strains were induced to express with iptg. the tannase activity was detected by ultravillet spectroscopy and the target protein was found in sds - page with the correct size of 63kd. the gene was also insered in the plasmid ppic9k of pichia pastoris
將單寧酶基因tan重組到trc啟動子控制下的表達載體pse380中，並分別轉化入大腸桿菌dh5 ， top10 ， bl21 （ de3 ）三種重組表達菌株中。 -
The gene encoding tannase was amplified from the total dna of aspergillus oryzae by pcr and was expressed in e. coli and pichia pastoris. the gene was insered in the expression plasmid pse380. the recombinant plasmid pse380 - tan was transformed into e. coli strains dh5a, top10, bl21 ( de3 )
本論文以米麴黴aspergillusoryzae的總dna為模板， pcr擴增了單寧酶基因（ tan ） ，並分別嘗試了利用大腸桿菌（ escherichiacoli ）和巴斯德畢赤酵母（ pichiapastoris ）進行表達。
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