taq dna polymerase 中文意思是什麼

taq dna polymerase 解釋
taq dna聚合酶
  • taq : 安其拉神廟
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • polymerase : n. 【生物化學】聚合酶。
  1. Among the three methods used in the experiment of dna extraction, only ctab, adding pvp in the dna isolation step, had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process. pcr amplifications were performed in a final volume of 25 mm3 containing 0. 5 units taq polymerase, 2

    通過在抽提緩沖液加入2的pvp 、並用異丙醇和乙醇沉澱基因組dna等改良措施, ctab法能避開大量纖維、多糖的影響,有效地從不同屬種的棕櫚科植物的幼嫩葉片中提取並純化了適合rapd的基因組dna 。
  2. That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers, then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting, and deposited by ethanol ). sequencing analysis of pcr product was done on 310 gene scan analysis ?. 1. 2 dna sequencer to determine telomerase activity. notably, this method eliminated the restraining factors of taq dna polymerase, making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results

    在kim等開發的端粒重復擴增分析法( trap )的基礎上進行改進,即通過對端粒酶延伸ts寡核昔酸反應產物的精製,消除了pcr擴增中抑制taq酶活性的因素,從而減少了樣品之間pcr擴增上的差異和假陰性現象的發生,提高了判斷樣品端粒酶陰、陽性的準確率和定量的準確性。
  3. Due to high sensitivity of rapd analysis to reaction conditions, main factors affecting the results including composition of the buffering system, concentrations of taq dna polymerase, primers and templates, and number of pcr cycle etc., were examined, and conditions applicable to rapd analysis of j. curcas were determined

    針對rapd標記影響因素眾多、結果重復性低的特點,對rapd分析中pcr擴增的各種條件進行了梯度測試,包括反應混合液成分、 taq酶量、引物濃度、模板濃度、 pcr循環數等。
  4. The y - a489 - plex multiplex pcr is feasible for using home product of taq dna polymerase

    Y一a489一plex體系採用的是國產的taq酶。
  5. Furthermore, the home product of taq dna polymerase had the same specificity and efficiency compared to the amplitaq gold ( pe, usa ) in this study. conclusion this is the first time that the tailed primer design protocol for multiplex pcr system is used for y - str loci

    初步構建了四個y一str基因座的y一a489一plex銀染體系和y一a4sg一plex熒光體系:並依據dna分析技術工作組( twgdam )指南進行了應用性研究。
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