target dna 中文意思是什麼
target dna
解釋
目的基因-
Dna sequence analysis indicated that tn5gusa5 is prone to insert into low gc content regions ; guanine is a preferential base at the first place and cytosine at the last site of target sequence
在質粒上tn5gusa5也傾向于插入低gc含量區；堿基g和c分別在靶序列的首位和末尾出現的幾率高。 -
Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly
結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
A chip based on electroelution principle was presented for the recovery of dna fragments, which can make the isolation and collection of target dna fragments possible in a single process by switching electrodes
提出了一種基於電洗脫原理的核酸純化回收晶元，通過對晶元上電極進行適當的切換操作，可一次完成核酸樣品分離和純化回收。 -
At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。 -
Study and primary application of the screening model for antituberculosis drugs with dna gyrase b subunit as a target
亞基為靶點的抗結核藥物篩選模型的建立及初步應用 -
Biotin - avitin system ( bas ) was used to immobilize dna on the pdms channel compared to the direct immobilization methds. the hybridization reaction also has been examined between immobilized probe and target dna
針對pdms新型材料，試驗了兩種dna分子的固定方法，確定了生物素?親和素介導的dna分子固定化方法，並使用此晶元和固定方法進行了晶元上的dna雜交反應。 -
After getting the cdna sequence of curcin, four fragments of the gene were cloned through pcr and high - level expression in e. coli was achieved. the four target dna fragments, i. e
在得到麻瘋樹毒蛋白cdna序列后，通過pcr對毒蛋白基因進行了克隆，並實現了部分基因片段在大腸桿菌中的高效表達。 -
A new resolution vector based on tnpi - mediated site - specific recombination system of b. thuringiensis transposon tn4430 was developed. the crylac10 or other target dna, and the gene ori1030, from a plasmid of wide type b. thitringiensis subsp
利用轉座因子構建解離載體的可行性利用蘇雲金芽胞桿菌轉座子tn4430的解離區構建了解離載體。 -
Since the complex cisplatin was clinically used in cancer chemotherapy in the late 1960s, metal complexes have attracted much attention as a new type of potential anticancer drugs involving dna as the major biological target
尤其是20世紀60年代末順鉑抗癌作用的發展和臨床應用，更進一步促進了以dna為靶分子的金屬抗癌藥物的研究。 -
Blood samples of the population of hans and uigurs were collected, genome dna extracted, and basis pcr conditions of amplifying target sequence established. 2
收集漢族、維吾爾族普通人群血標本，提取白細胞基因組dna ，並建立擴增目的基因片段的基本pcr反應體系。 -
This research has closed relationship with a wide range of cross - science areas, such as basic research on cancer therapy by heavy ion radiation method, radiation danger level evaluation in space and biological damage induced by long term, low - level dose radiation environment, etc. dna is the carrier of biological information and the main target of biological effects induced by ionizing radiations
從重離子治癌的基礎性和先導性的研究，載人航天飛行過程中的太空輻射危險性評估，到長期在低劑量輻照環境下的放射性對機體損傷等等，都與電離輻射所致生物損傷的研究有著密切的關系。 dna （脫氧核糖核酸）是生命信息的載體，也是輻射生物效應的最主要的靶分子。 -
In order to understand the mechanism of mtx further and to investigate the genotoxic target organs, we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay. liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at lh, 3h, 6h, 12h, 24h after 5mg / kg mtx intraperitoneal injection
為了進一步了解甲氨蝶呤（ mtx ）的作用機制，探測其作用的遺傳毒性靶器官，為應用mtx治療過程中的臨床監測和副作用防治提供理論依據，我們以小鼠為研究對象，用單細胞凝膠電泳技術檢測了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后對肝、脾、骨髓、胸腺、腎、睪丸、胃和外周血淋巴細胞的dna損傷作用及損傷程度與mtx劑量間的關系。 -
But up to new, little is known about the mechanism of the antirumoural activity of organogermanium compounds, as for cisplatin and organotin, dna was proposed to be the target, and little work has been done on the interaction between organogermanium and dna or its precursors
本試驗通過紫外吸收光譜、熒光光譜研究了無機鍺（以二氧化鍺為代表）和有機鍺（以二氯二乙基鍺為代表）與dna的相互作用的光譜變化，並對二者相互作用的最適外界環境作了較為詳細的研究。 -
Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene
反義核酸技術是根據堿基互補原理，使用與目標靶的遺傳物質（ mrna或dna ）特定互補的核酸片段封閉基因表達的技術方法。 -
Addition of iptg to growing culture of the lysogen induces t7 rna polymerase, which in turn transcribe the target dna in the plasmid. in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg, a 52kd protein with tryptopanase activity was expressed
摸索發酵條件，如改變培養溫度和iptg濃度等，發現在30培養條件下， 0 . 2mmiptg誘導時，發酵液中的吲哚含量最高，表明低濃度的誘導劑或低溫誘導有利於表達出有活性的色氨酸酶。 -
The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr, and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5為標簽，用錨定pcr法克隆出突變株nm4的tn5側翼序列。並以此側翼序列為標簽繼續用錨定pcr法向兩邊延伸，克隆出一個5045bp的dna片段。 -
The scientists combined two approaches for analyzing genetic information. one approach scanned the entire human genome for suspicious areas of dna while the second approach closely examined specific target genes
科學家聯合兩種科研方法來分析遺傳信息。使用的一種方法掃描了全基因組以尋找懷疑的dna區域，另一方法則用於檢測特異的靶基因。 -
T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
轉基因芥菜植株的鑒定取再生苗的葉片提取dna ，進行pcr擴增和pcrsouthernblot分析，轉化再生植株大部分呈陽性，而非轉化的再生植株均為陰性，證明cpt基因已存在於芥菜基因組中。 -
2. to construct the prokaryotic expression vector and the control vector. after sequenced, the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
將自puc18 h d - 3回收的目的片段連同表達運載蛋白dhfr的基因片斷一起連接于原核高效表達載體pqe - 80l ，經酶切鑒定，得到重組原核表達載體pqe - 80l dhfr h d - 3 。
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