target fragment 中文意思是什麼
target fragment
解釋
靶片斷-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
The edge detection approach based on cp neural network is valid to overcome the randomness of fragment dispersing and complexity of distribution of perforation holes. applying neural network approach, the influence of the negative factors is reduced in artificial detection and statistics. in this way, the target image was detected exactly and successfully
基於cp神經網路的邊緣檢測方法有效地克服戰斗部破片穿靶試驗中破片飛散的隨機性和破片孔分佈的復雜性，從而減小了人為檢測和統計中諸多不利因素的影響，完成對靶板圖像的準確檢測。 -
The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector
根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。 -
In this thesis, the neural network and edge detection technologies were reviewed firstly, then discussed the application in weapon engineering, and illuminated the features and significances of the study. secondly, the main algorithms and its application relate to edge detection were summarized, and then the statistics theory and target edge detection based on neural network was discussed in detail, referred to fragment warhead. moreover, the edge detection method based on cp neural network model and technologies was given, through which the target images were detected exactly
文中首先詳細論述神經網路及邊緣檢測技術的發展及在武器工程領域的應用，闡明本研究課題的特點及實用價值;然後，對邊緣檢測技術所涉及的主要演算法特點及其應用技術進行了全面的概括論述;接著詳細論述破片戰斗部所涉及的相關數學統計理論及基於神經網路的目標靶板邊緣檢測技術，提出一種基於cp神經網路模型及技術應用的實現方法，完成對目標靶板試驗圖像的邊緣檢測;最後，對神經網路對目標靶板的檢測方法和人為檢測目標靶板方法進行了比對及總結，論證了神經網路檢測方法的可行性和先進性。 -
The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr, and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5為標簽，用錨定pcr法克隆出突變株nm4的tn5側翼序列。並以此側翼序列為標簽繼續用錨定pcr法向兩邊延伸，克隆出一個5045bp的dna片段。 -
To answer this question, a bispecific, trifunctional antibody constructs which can not only target block virus incorporated rca, but also can induce complement activation by it ' s fc fragment were designed and constructed and iv the role of this kind of bispecific antibody in virus neutralization was studied. 1. to test our idea, human immunodeficient virus ( hiv ) and enveloped extracellular virus ( eev ) of vaccinia virus ( vv ) were selected in our study because of their complex immune evasion stratiges, their threaten to humans, and because both these two kinds of virus can escape complement attack by incorporating host rca into their envelope
以嚴重危害人類健康，且具有復雜免疫逃避機制的有包膜的hiv病毒及痘苗病毒的eev病毒為研究對象，首先對它們逃避補體攻擊的現象進行了驗證，探討了宿主膜補體調節蛋白cd55 、 cd59與hiv病毒及eev病毒免疫逃避的關系，評價了病毒結合的這兩種補體調節蛋白作為本研究提出的，通過消除病毒逃避補體攻擊的機制來恢復病毒對補體攻擊的敏感性，提高補體抗病毒效率這一抗病毒策略的靶點的可能性。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。 -
2. to construct the prokaryotic expression vector and the control vector. after sequenced, the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
將自puc18 h d - 3回收的目的片段連同表達運載蛋白dhfr的基因片斷一起連接于原核高效表達載體pqe - 80l ，經酶切鑒定，得到重組原核表達載體pqe - 80l dhfr h d - 3 。 -
Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh, the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form. both of the products in the two strains showed biological activity, but the former is 2 times higher than the latter. the hyuc dna fragment was inserted into ppic3. 5k plasmid to construct the ppic3. 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion
結果表明， sds - page分析在50kd處有一較強的表達帶，融合有分子伴侶的重組菌株bl21 （ de3 ） pt221 - hyuh與非融合表達的重組菌株m15 pqe60 - hyuh相比，乙內酰脲水解酶的表達量低一些，但其表達蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表達蛋白則主要以包含體形式存在，且前者的酶活性是後者的2倍多。 -
The target fragment cbf3 was obtained after plasmid pcbf3 having been digested by bgll ii, which correctly inserted into linetype vector that was obtained after pvct2011 had been digested by bamhi, then middle vector pvct2012 containing rd29a - cbf3 - tnos gene expression box was constructed
質粒pcbf3經bgllll單酶切后獲得的目的基因片段cbs正確的插入到pvct2011經bwhl單酶切后的線型載體中，構建成攜帶有rd29a cbf3 tnos基因表達盒的中間載體pvct2012 。 -
A sobel edge operator and an edge detection algorithm based on cp neural network was employed in this thesis, to achieve image processing of target digital images collecting from static explosion test of fragment warhead, and exactly detecting and analyzing of target fragments numbers, and also the ratio of perforation of fragments from the test, through which more accurate tactics performance characters can be obtained for warhead lethality assessment
本文採用sobel邊緣運算元和一種基於cp神經網路的邊緣檢測方法，對破片戰斗部靜爆試驗后所採集的目標靶板數字圖像進行了圖像處理，實現了對目標靶板破片數量及破片穿透率試驗統計數據的準確檢測與分析，為破片戰斗部殺傷威力評估提供了較為精確的技戰術指標。 -
The technique is applicable for target detections of a variety of fragment warhead test
該技術將應用於各種破片戰斗部試驗目標靶板檢測中。 -
The main difference is that a fragment does not have a plug - in class - the fragment s life cycle is managed by its target plug - in
主要的區別就是片段沒有插件類片段的生命周期由其目標插件管理。
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