terminal protein 中文意思是什麼
terminal protein
解釋
末端蛋白-
Cloning and identification of chicken and pig bpi protein n - terminal fragment
氨基端的基因克隆和鑒定 -
Results : after cryofixation, basement membranes of skeletal muscle consisted of only one electron dense layer, t tubules were round, core cylinders were observed in terminal cisternae and there were thread - like protein particles on the membranes of terminal cisternae
化學固定后，縫匠肌基膜由兩層組成：一層電子密度低，另一層電子密度高；橫小管為扁平狀或啞鈴狀；終池內僅有一些散在的電子密度高的顆粒，終池膜上有幾個腳狀突起伸向橫小管。 -
The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
In the 1990s, the pheromones of gram - positive bacteria, which regulates the growth and toxin secretion of the same type bacteria, were identified they were peptides consisted by dozens of amino acids. the pheromones can auto - recognize membrane receptor of the identical types of bacteria. we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia
丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白，命名為pheromonicin ，該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。 -
Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis
Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。 -
The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。 -
The three isoforms have a common carboxy - terminal domain of 188 amino acids, and this region is highly homologous to the reticulon protein family
在nogo分子的c -末端，有兩個跨膜片段，被66個氨基酸殘基組成的環狀結構（ nogo - 66 ）所分隔。 -
Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。 -
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method
Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。 -
We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization
我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。 -
In vivo, the two functional forms of rat mapllc3 with apparent mobilities of 18 and 16kda, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved glyl20 residue and this cleavage is essential for the membrane association of the 16kda rat map1lc3 protein.,
其中18kda的map1lc3蛋白是微管相關蛋白1的輕鏈亞基，稱為lc3 - ；另一種16kda的map1lc3蛋白則是自噬體膜的必須組分，稱為lc3 - 。 lc3 -和lc3 -都是一系列翻譯后修飾的產物，其中特徵性的修飾是在保守gly120后發生的羧基端切割，此切割修飾對lc3 -定位於自噬體膜尤為重要。 -
In vivo, the two functional forms of rat map1lc3 with apparent mobilities of 18 and 16kb, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved gly120 residue and this cleavage was essential for the membrane association of the 16kd rat mapllc3 protein
Lc3 ?和lc3 ?都是一系列翻譯后修飾的產物，其中特徵性的修飾是在保守gly120后發生的羧基端切割，此切割修飾對lc3 -定位於自噬體膜尤為重要。我們研究發現在大鼠，小鼠和人中map1lc3主要以兩種型存在， lc3a型和lc3b型。 -
An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells
利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。 -
The ir sequence of mxmybl is most homologous with that of atmyb ( 86. 9 % ) and somewhat homologous with that of stmyb 1 ( 77. 0 % ) ; there is very low homology among n - and c - terminal regions outside of the ir regions of all of the mybs ; the protein mxmybl contains a proline - rich region as well as a serine - rich region near the c - terminus, such structure motifs are implicated in transcriptional activation
9 ，與馬鈴薯stmyb的ir序列的同源性達77 0 ，所有這些nnrb蛋白除了瓜區具有較高的同源性外，其c端和n端幾乎沒有同源性。 mwyb蛋白的c一端還含有一個富含脯氨酸區，這樣的結構基序可能具有激活轉錄的功能。 -
The polyurethane ( pu ) membranes modified with silk fibroin ( sf ), made from sf protein and liquid prepolymer with terminal - isocyanate groups, were obtained by the process of prepolymer having reaction on the surface of sf membranes and then controlling the moisture of system and the solution conditions
摘要以再生絲素蛋白和液狀端異氰酸酯基預聚物為原料，使預聚物在絲素膜界面發生化學反應，再通過控制相對濕度和溶解條件，制備了絲素改性聚氨酯膜。 -
The protein accumulated mainly in secondary phloem parenchyma cells and secondary phloem ray cells. the degradation of storage protein in terminal branchlets of swietenia macrophylla synchronized with new shoot growth after leaf - absent period, suggesting that the protein was utilized to support the growth of new shoots. when the diminishing of the storage protein below the girdled site was blocked, the new shoot growth was also restrained
在樹木新的年生長周期中，隨著新梢的發育，積累在末端小枝中的貯藏蛋白質開始被消耗新梢葉片成熟時，末端小枝中的18kda和21kda蛋白質己完全消失，而樹乾和大根中的這兩種蛋白質的含量相對穩定，與落葉期相比幾乎沒有變化。 -
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。 -
The predicted vfcpkl protein with 493 amino acids contains all the structural characteristics of reported cdpks and there are four domains of a variable domain, a kinase domain, an autoinhibitory domain and a calmodulin - like domain from n - to c - terminal end
由編碼區推測的vfcpk1的493個氨基酸序列具有已報道的cdpk的所有典型結構特徵，由n端到c端可分為可變區、激酶區、連接區和調節區四個結構域。 -
Mass spectrometry technique will play more and more role in the field of sequence analysis. standard amino acid thiohydantoins are required as reference standard for development of c - terminal protein sequencing based on the thiohydantoin procedure
在蛋白質c端（異）硫氰酸法順序測定技術中，標準氨基酸乙內酰硫脲（ th - aa ）的制備是必須首先要解決的問題。 -
Among numerous strategies for c - terminal protein sequencing ( iso ) thiocyanate method established by schlack - kumpf in 1926 appears to be a promising approach because of the similarity to the edman degradation in mechanism
縱觀蛋白質c端測序方法的發展，由於化學法中的（異）硫氰酸法反應機理與edman降解十分類似，方法，所以是最有前景的方法。
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