tgev 中文意思是什麼

tgev 解釋
傳染性胃腸炎病毒
  1. Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified

    為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。
  2. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。
  3. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  4. After the estimation of labeling efficiency tp was applicated to hybridize the rna of tgev, then was subjected to self - development of x - ray exposed by cspd to show the target nucleic acid

    對標記效率測定后，以帶正電的尼龍膜為介質進行特異性和靈敏性檢測，經非放射性自顯影來顯示靶核酸。
  5. The genome structure of tgev, pedv and prcv had high homology and it was difficult to diagnose tgev by traditional means. two studies were carried out to differentiate the tgev from prcv and investigate the epidemiology of tge

    Tgev與豬流行性腹瀉病毒（ pedv ） 、豬呼吸道冠狀病毒（ prcv ）在臨床癥狀上很相似，基因組結構上與tgev具有較高的同源性，傳統的方法很難鑒別。
  6. The anibodies to n protein of tgev could be detected in the serum of mice from group b at 39 days of pi

    B組小鼠在首次免疫后第39d檢測到針對tgevn蛋白的陽性抗體（ p / n 2 . 0 ） ，其它時間均未檢出陽性抗體。
  7. Nonradioactive nucleic probe to tgev was prepared and was applicated to detect tgev at first stage, which established foundational work for differentiating tgev from prcv and investigation on epidemiology of tgev

    制備了tgev非放射性標記的核酸探針，並初步應用於檢測tgev 。二者為tgev鑒別診斷、血清學調查奠定了基礎。
  8. Spike protein was the main protective antigen inducing the neutralization antibody. the fragment containing antigenic sites b and c locating at the 5 " terminus in spike gene of tgev was deleted in prcv

    Tgevs蛋白可誘導產生中和抗體，是主要的保護性抗原，其n端抗原位點b和c在prcv中缺失。
  9. Transmissible gastroenteritis ( tge ) was an enteric disease of pigs caused by transmissible gastroenteritis virus ( tgev ), a member of coronaviridae family. tgev was characterized with high mortality and had spread all over the world

    豬傳染性胃腸炎病毒（ transmissiblegastroenteritisvirus ， tgev ）是冠狀病毒科、冠狀病毒屬成員，引起豬的傳染性胃腸炎（ transmissiblegastroenteritistge ） ，死亡率很高，呈世界范圍分佈，嚴重危害養豬業的發展。
  10. The rabbits were haintmed with purified recombinant protein and the sera were examined for anti - tgev neutralwi astivity on the continuous porcine ibrs2 cells. the result showed that neutraldrig activity was 1 : 22. therefore, the neuthelizing activity of anti n protein antibody against infectious tgev in vitro was very iow from the result of neutra1hation ( vn ) test

    將純化的融合蛋白免疫家兔，制備的抗血清在豬ibrs _ 2傳代細胞上進行中和實驗，結果顯示中和效價為1 ： 22 ，表明n蛋白所產生的抗體對tgev的中和活性較低。
  11. The minimal amount of tgev cdna can be detected was about 120 pg in this research

    本研究中探針tp檢測到tgevcdna最低量為120pg 。
  12. The 285 bp fragment ( named tp ), locating at 5 ' terminus of spike gene, had high conservation in tgev isolations

    Tgevs基因5 』端285bp片段在tgev分離株中較為保守，與其它冠狀病毒同源性較低。
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