thioredoxin 中文意思是什麼

thioredoxin 解釋
硫氧還蛋白
  1. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  2. Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

    由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。
  3. The other is ferredoxin / thioredoxin system located in nonphotosynthetic tissue and cytosol of photosynthetic cell, which includes ferredoxin, ferredoxin dependent thioredoxin reductase, thioredoxin h. this two system constitutes important cellular redox regulatory system and can regulate the redox intercellular environment, metablism and signaling transduction

    另一個是nadp （ h ） -硫氧還蛋白系統，該系統是由h -型的硫氧還蛋白、 nadp （ h ） 、和依賴于nadp （ h ）的硫氧還蛋白還原酶（一種黃素蛋白）組成。
  4. Changes in proteins within germinating seeds of transgenic wheat with an antisense construct directed gainst the thioredoxin

    轉反義硫氧還蛋白基因小麥萌發種子中蛋白質的變化
  5. Depending on cellular location and function of thioredoxins, there are two thioredoxin systems in high plants, one is nadp / thioredoxin system mainly existing in chloroplast of high plant and eucaryote algae and oxygenic photosynthetic prokaryote, which is composed of nadph, nadph - dependent thioredoxin reductase, thioredoxin - f and thioredoxin - m

    在高等植物中，存在兩個與硫氧還蛋白密切相關的硫氧還蛋白系統：一個是位於葉綠體中的鐵氧還蛋白-硫氧還蛋白系統，該系統中含有兩類核基因編碼的硫氧還蛋白即f -型trx和m -型trx 。
  6. A web - based blast is also constructed to facilitate the process. and the contigs and ests sequences are formatted as subject database. by similarity search, about 442 estss were found possibly encoding immune related proteins, such as lysozyme, lectin, serine protease, serpin, thioredoxin, sod and so on

    為了方便相似性比對，在由四臺pc機并行組成的機群（ pc - cluster ）上將blast程序和interproscan程序本地化，並且將contig和ests序列文件做成blast程序中的序列庫文件以供搜索。
  7. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。
  8. The total sequence is 663 basepairs with an open reading frame of 354 nucleotides encoding a novel h - type thioredoxin and an utr of 37 basepairs at its 5 " end and an utr of 279 basepairs at its 3 " end

    通過對其序列特徵、基因結構和在鹽脅迫下的表達特徵分析得知tstrx全長序列為663bp 。其5端的utr含有37個堿基，其3 utr含有272個堿基，該序列中含有一個編碼118個氨基酸的開放閱讀框。
  9. It is the foundation of therapy of hepatitis b. the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll. the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures

    融合硫氧還蛋白的單鏈抗體在大腸桿菌bl21 （ de3 ）中以包涵體的形式得到高效表達，單鏈抗體表達量達佔30的總蛋白以上。
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