tissue specific antigen 中文意思是什麼
tissue specific antigen
解釋
組織特異性抗原-
The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300
二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
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