tnfa 中文意思是什麼

tnfa 解釋
噴嘴總過流面積
  1. Recently, some new studies suggest that small cytokine peptide - mimics can function as the agonist or antagonist of cytokine receptor, and these small molecular peptides can be synthesized easily and oral adminstration with high concentration. based on these work, we have tried to set up experiment on tnfa - binding peptides and tnfa mimotopes which may be as leads of tnfa antagonist

    近年來的研究發現，小分子細胞因子模擬物具有細胞因子受體激動劑或拮抗劑的作用，且小分子模擬物因容易合成並能夠給予高濃度、可口服等優點使其具有更加廣闊的研發與應用前景。
  2. It is well known that tumor necrosis factor a ( tnfa ) can play very different character in defence mechnism of body and pathologic injury, in which it can inhibit or kill tumor cell, and induce inflammation against infection ; on the other hand, tnfa can be as a very important mediator to cause some serious pathologic processes such as septic shock, gvhr and some autoimmune diseases

    腫瘤壞死因子（ tumornecrosisfactor ， tnf ）具有對某些惡性腫瘤的抑制或殺傷作用，也是致內毒素性休克、急性移植物抗宿主病及一些自身免疫病的重要效應因子，因此tnf的改造和tnf拮抗劑的研究受到各國學者的重視。
  3. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  4. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  5. These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa

    3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立：為了深入研究細胞因於與配基（受體或抗體）相互作用的關鍵殘基及其空間構象，在上述研究基礎上，我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫，找尋與之結合的tnfa結合肽噬菌體克隆；並以此拓寬了噬菌體肽庫的應用范圍。
  6. Part iv computer - aided molecule modelling of tnfa mimotopes and tnfa - binding peptides : to investigate the interaction between tnfa mimotopes and tnfa - binding peptides, the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt - 18 with tnfa firstly, then lcs - 7 was docked to llt - 18 by manual. the interaction between llt - 18 and tnfa or lcs - 7 showed electrostatic interaction and h - bond dominant

    進行對接，對兩者間結合位點進行分析；在此基礎之上，以insight11軟體構建lcs刁分於三維結構， autodock程序對接llt和lcs 7兩個短肽分子，找尋兩者結合的關鍵性殘基，結果表明llt與tnfa及lcs 7分子間相互作用以靜電相互作用為主， lcs刀和tnfa活性部位的arg在分于間相互作用中起重要作用。
  7. After 3 rounds of screening, 10 of 20 clones were identified as positive clones and all the 10 phage clones sharing the same amino acid sequence : ehmaltypfrpp, and these positive 12mer phage clone peptide can bind with tnfa specifically. the results suggests that this method is very specific and simple for screening of binding epitope to small peptide molecule from phage display peptide library

    在以噬菌體環七肽tnfa模擬肽克隆刁k naqsoc ）為靶對噬菌體隨機十二肽庫進行h輪篩選后，挑取20個克隆，經elisa鑒定有10個克隆均與t 』 nfa結合，測序結果顯示它們均為同一氨基酸序列： ehmaltypfrpp 。
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