transfer blocking 中文意思是什麼
transfer blocking
解釋
轉換上盤-
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
Vcd hk version - it is a full screen transfer, with medium image and sound qualities. the english subtitles are decent with very few grammatical errors. but somehow the subtitles appear so big on the screen, blocking at least 1 5 of the full screen image
但看來一般觀眾也只是以獵奇的心態去觀賞一連串的殺人場面，集中焦點在暴力血腥之上，而未有餘暇探索當中奧妙，會回頭反思的觀眾就更少，看來深作欣二的苦心都是白廢居多。 -
On the basis of analyzing information intention in a general meaning, systematically defining the extension scope of quality ' s concept, and researching of the division as well as establishment of the whole process ' s quality ring, it delves into the formation of product quality, the dynamic characteristics of product quality information, and builds the framework of quality information, nine implementation links of quality information transfer, and three function modules. and in the end, taking the analysis of information channel ' s distortion and noises in the general mode of information transfer, it puts forward ways to solve the " blocking - noise problem " in the transfer process of quality information in the respects of origin, channel and destination of information
以一股意義上的信息內涵分析和系統地對質量概念外延范圍的界定和劃分、建立全過程質量環的研究為基礎，詳細考察了產品質量的形成、產品質量信息的動態特徵，構建了質量信息框架、質量信息傳遞的九個實施環節和三個功能模塊，最後以一般性信息傳遞模式中通道失真與噪聲分析為基礎，提出從信源、通道、信宿三方面解決質量信息傳遞過程中「阻噪問題」的思路。 -
Juve are seemingly keen on blocking the duo now, in order to finalise a transfer in june, whereas the rossoneri might decide to make an immediate offer to bring cavani to the san siro this month
尤文現在表面上對這對雙子星很熱衷，希望能在六月份完成轉會，然而紅黑軍團可能決定在這個月就開價將卡瓦尼帶到聖西羅
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