transformation strain 中文意思是什麼

transformation strain 解釋
轉變應變
  • transformation : n 轉變,變化;變形;【生物學】(尤指昆蟲的)轉化,變態,改造,改革;變質;【數學】變換;【電學】...
  • strain : vt 1 用力拉,拉緊,抽緊,扯緊。2 使緊張;盡量使用(肌肉等)。3 強迫,強制;濫用,盡量利用。4 拉傷...
  1. The result of using eight kinds of different yeast strain as biocatalyst reveals that baker ' s yeast is the best biocatalyst in this transformation

    比較了八種酵母的生物轉化能力,發現在有機合成生物轉化中應用最廣泛的生物催化劑baker ' s酵母最適合催化該模型反應; 3
  2. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌,提取初提物中的總蛋白,進行sds一聚丙烯酞胺凝膠電泳,檢測表達的融合蛋白大小越為gokd 。
  3. This thesis briefly analyzes some yield criteria and instability criteria to the sheet metal forming first. then according to the transformation rule from ultimate strain to ultimate stress, it establishes the mathematical calculation models of the forming limit stress diagram for the software development according to hill48 、 hill79 and hosford yield criterion irrespectively

    本文首先分析了板料成形的屈服準則和失穩準則,然後根據極限應變到極限應力的轉換關系,分別採用hill48屈服準則、 hill79屈服準則和hosford屈服準則,建立了用於軟體開發的成形極限應力圖的數學計算模型。
  4. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌后,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。
  5. The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas

    對出發菌株ybt - 1463和其無質粒突變株bmb171的部分形態、生理生化和遺傳學特性進行的比較研究的結果表明,突變株bmb171不形成伴胞晶體,但在個體形態與菌落特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發菌株ybt - 1463無明顯差異。
  6. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗,檢測目的基因的整和插入;提取總rna ,進行了northern實驗,檢測fl基因在轉化菌株中的表達。
  7. The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern

    通過實驗和對結果的數據分析,得出下面的結論:發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株,它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率( 60 ) ,最適的菌株感染濃度od _ ( 600 )為0 . 7 ,溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養,以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。
  8. Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants, agrobacterium rhizogenes, affecting factors and their magnitude and the underlying mechanism in the transformation, therefore to determine the optimum explants, pre - culture time, tested strain concentration, infection time, co - culture time, affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test, this project was launched. with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants, agrobacterium rhizogenes r1601 and r1000 as infection agents, transformation was performed using different methods and under various circumstances

    為了建立一種有效的誘導大苞栝樓發根的方法及其發根培養體系,以適合於發根生物反應器擴大培養研究和應用基礎的需要;本研究對誘導發根的外植體在發根農桿菌和環境因子等影響作用下進行發根的誘發及其可能的機理進行探討,從而找出用於發根誘導和培養的最佳外植體、預培養時間、發根農桿菌濃度、感染時間和共培養時間、誘導條件,並通過正交試驗得出優化的誘導條件。
  9. 5 ) as the deformation strain increases the reverse transformation temperature and thermal hystersis of tini film increase on the first heating, while the martensitic transformation temperature decreases and r phase transition temperature is constant

    隨預應變量增大, tint薄膜的第一次逆相變溫度升高,相變熱滯增大,馬氏體相變溫度降低, r相變溫度無明顯變化。
  10. The result showed that plastic deformation during pre - strain played an restrained role on the growth of martensite ( especially in the direction of lath width ) and increase the nucleation rate indirectly ; the elastic energy induced by plastic deformation restrain the transformation of primary martensite but accelerate the transformation of after martensite

    實驗結果表明:預應變過程中造成的塑性變形對馬氏體生長(尤其是寬度方向)有約束作用,起間接提高馬氏體形核率的作用,所儲備的彈性應變能對初生馬氏體的轉變有抑制作用,但對繼生馬氏體的轉變卻有促進作用。
  11. It may be due to active fracture and seismogeny procedure, and may be due to microdynamic activity of regional strain energy can accumulate or release in different stages, and there exists transformation process of stress

    重力場變化可能與區域應力應變場微動態活動有關,地殼運動過程中存在著區域應變能積累和釋放調整階段,並存在應力傳遞過程。
  12. With the method of the lie group transformation, the symmetry of the equation governing one dimensional finite strain consolidation is discussed and, from the point of the symmetry, the feasibility to obtain the analytical solution of these nonlinear partial differential equations is discussed. where - after exact or approximate analytical solutions focused on different consolidation problems are obtained, these including : under some assumptions of relations of the void ratio with coefficient of permeability and effective stress, the method of lie group transformation is applied to solve the non - linear partial differential equation of large strain consolidation of homogenous saturated clay soil in semi - infinite domain with the consideration of the material and geometrical nonlinearity during consolidation procession. the implicit exact solution without considering the effect of self - weight of soil is obtained

    運用lie群變換方法討論了一維大應變非線性固結方程的對稱性,以及在該對稱性的意義下求解這類非線性偏微分方程解析解答的可能性,並就大應變非線性固結問題的多種情況求得了其完整的或者近似的解析解答,具體包括:基於有效應力與孔隙比以及滲透系數與孔隙比之間的關系的一些假定,採用李群變換求解考慮材料非線性和幾何非線性的半無限均質土體大變形固結非線性偏微分方程,得到了一個不考慮自重固結的完全解析解。
  13. The effect of plastic deformation and elastic strain energy induced by pre - strain on the nucleation of martensitic transformation of low alloy ultra - strength steel sosimncrmov had been studied by pre - strain quenching in austenite state. the control over deformation magnitude and deformation temperate as well as loading pattern was performed to adjust the storage of elastic strain energy in steel before martensitic transformation, using the type of geeble - 1500 thermal analogue test machine

    使用gleeble - 1500型熱模擬機,對低合金超高強度鋼30simncrmov實施奧氏體預應變淬火處理,通過控制形變量、形變溫度和加載方式,調整鋼中馬氏體相變前的彈性應變能儲備,研究了預應變所造成的塑性變形與彈性應變能對馬氏體相變的影響。
  14. The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied

    通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上,分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ,並分別導入無質粒突變株bmb171 ,篩選得到攜帶相應icps基因的重組菌株。
  15. The weigh - in - motion system of vehicle, including the structure and dynamic characters of the strain sensors, the hardware structure of signal sampling - amplifying and a / d transformation and the precision & sensor space design expression of sample mean approach was designed

    摘要設計了基於應變式稱重傳感器的車輛行駛稱重系統,包括應變式稱重傳感器的結構特點、動態特性、信號採集放大與模數轉換的硬體結構,以及基於采樣平均法的信號處理精度和傳感器間距的設計表達式。
  16. 2 establishment of genetic transformation system by agrobacterium rhizogenes the different types of explants ( hypocotyls, cotyledon, leaf blade, petiole ) were infected by strain r1601 of agrobacterium rhizogenes

    ( 2 0mg l )上培養30d后經過體細胞胚胎發生途徑產生大量的叢生苗;子葉培養在誘導子葉產生叢生苗培養基( msoc ) ms ba ( 2 0 。
  17. The characteristics of different boundary conditions are evaluated. by applying the approach of the transformation of lie group, the exact analytical solutions of the non - linear consolidation equation is obtained under different initial and boundary conditions. based on this solution, the differences between that of large and infinite strain consolidation theories are analyzed

    運用變換群的方法求得了某些條件下大應變固結方程的完整解析解答,在此基礎上與相同條件下的小應變固結論理做了比較,並通過對實例的計算,分析了不同假設條件下一維固結理論之間的差異。
  18. By the same method, the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion. after that, the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method. the pcr amplification indicated the lba4404 strain containning cpti gene. the lba4404 strain was used in genie transformation of mustard

    經酶切和pcr擴增驗證后,以xbai和saci雙酶切pgem ? cp和pbi121 ,將切下的cpti片段和pbi121載體片段連接構建成表達載體pbi121 - cp ,用凍融法導入農桿菌lba4404 ,提取質粒,經pcr擴增檢測,用於芥菜的遺傳轉化。
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