trka 中文意思是什麼
trka
解釋
特爾卡-
In our study, the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones
我們將trk受體膜外域1911結構域與gal4蛋白融合構建成pgbkt7一ign ，確認無自激活作用后，將其作為誘餌蛋白，洲于酵母雙雜交篩選。 -
2. the functional of the purified trka extracellular domain proteins were tested using pc - 12 cells
2 、用pc12細胞株測定腸ka膜外域各結構域重組蛋白的功能。 -
The sds - page analysis revealed that the trka extracellular domains proteins were highly expressed and accumulated up to above 30 % of the total bacterial proteins after iptg induction
稱a膜外域結構域重組蛋白表達量均佔全菌蛋白的30 %以上。 -
It suggests that the second ig - like domain of the trka receptors is critical for ngf binding and receptor activation
表明i夕在介導ngf與腸沁、的功能結合過程中發揮著至關重要的作用。 -
Ngf binding to trka receptors results in receptor dimerization and kinase activation. recent evidence supports that not all extracellular subdomains are responsible for receptor activation. structure based drug design for neurotrophic agonists with small molecular weight relies on knowledge of the interaction of neurotrophin with their receptors
Trka在ngf作用下發生二聚化，使胞內域中酪氨酸激酶區激活，從而使trka中酪氨酸自磷酸化，並進一步激活胞內信號轉導通路，從而實現神經營養信號傳遞。 -
Trka, the high affinity ngf receptor, contains an intracellular domain with tyrosine kinase activity and an extracellular domain with three tandem leucine - rich motifs flanked by two cysteine clusters in their amino termini and two immunoglobulin - like domains in the more membrane - proximal region
膜外域含有8個亮氨酸的重復區域，其前後分別串聯著一組半胱氨酸殘基，緊挨細胞膜的是兩個c2 -型免疫球蛋白樣區ig和ig ；膜內域為酪氨酸激酶催化活性區。 -
However, this strategy lacks the ability of high - throughput screening. as the random screening approach requires a target molecule, it is essential to identify the subdomain of trka responsible for ngf binding and receptor activation
傳統觀點認為酪氨酸蛋白激酶型受體由於有較大的膜外域，小分子化合物難以使其激活，也難模擬大分子的生物的效應。 -
In present study, the recombinant subdomains of trka were expressed and purified. pc 12 cells were used to evaluate their ability to inhibit the neurite outgrowth induced by ngf
這研究，將為深入闡明， 11k受體配體識別的分子與結構機制以及研製具有藥用價值的先導小分子化合物打下堅實基礎。
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