tryptophanase 中文意思是什麼
tryptophanase
解釋
色氨酸酶-
The presence of calcium is beneficial to obtain higher tryptophanase activity. the activity of tryptophanse expressed by addition of appropriate concerntration of iptg is 91 - fold higher than that of no induction. the highest specific activity was
0 . 2mmiptg對菌體的生長有抑制作用，在細胞對數生長期前期誘導抑制最強烈， 0 . 1mmiptg基本不抑制生長；臨界濃度0 . 15mm的iptg和37ac是比較好的組合。 -
Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase. the fermentation conditions were optimized respectively. in the presence of glucose and iptg as induce agent, the concentration is the crucial factor for expression of active tryptophanase. if the iptg concentration is less than 0. 2mm, the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg
用iptg誘導表達實驗結果表明：利用t7啟動子在胞內能表達出有活性的酶； iptg與溫度的合適組合可以得到較高活性的色氨酸酶，在0 . 2mm以下濃度的iptg誘導時， 37是最適溫度，而用較高濃度的iptg誘導，低溫是表達有活性的色氨酸酶的必要條件，而且誘導時間要短。
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