whole serum 中文意思是什麼
whole serum
解釋
全血清-
One profile was obtained for whole serum.
其中一個圖譜是為全血清作出的。 -
Methods serums containing whole wmt and its disassembled formulas, including the formula consisted of warming jing and boosting qi part wenjin yiqi, wy and that of promoting blood circulation part huoxue tongmai, ht, as well as the serum contained high concentration of lipids were prepared conventionally, respectively. the adhesion of monocytes cell strain thp1 to human umbilical vascular endothelial cells huvec was determined by rose bengal stain method, and elisa was used to detect expressions of intercellular adhesion molecule icam1, vascular cellular adhesion molecule vcam1 and p selectin on huvec surface
方法常規制備溫脈通全方溫經益氣拆方活血通脈拆方含藥血清和高脂血清，採用虎紅染色法檢測藥物血清對高脂誘導的人臍靜脈內皮細胞huvec和單核細胞株thp1黏附的作用用細胞elisa法檢測huvec表面細胞間黏附分子1 icam1血管細胞黏附分子1 vcam1 p選擇素pselectin的表達。 -
But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose
對分離到的血清用飽和硫酸銨鹽析法提取igg ，並用deae纖維素離子交換層析法對提取的igg進行純化。 -
Standard practice for testing for whole complement activation in serum by solid materials
用固體材料測試血漿中整個活性的標準操作規程 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。 -
In contrast, in control gerbils or gerbils were unsuccessfully challenged, neither h. pylori nor other urease - positive organisms could be cultured from the gastroduodenal mucosa, rut results were consistently negative, and no serum antibodies to h. pylori whole - cell sonicates were detected
Pylori快速尿素酶檢測、血清學抗h pyloriigg抗體檢測和特異性pcr檢測，結果表明h pylori感染組動物以上各項指標均為陽性，表明已經感染h
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