wild-type strain 中文意思是什麼
wild-type strain
解釋
野生型菌株-
A strain of the mutant mgxcr1 with a much lower level of cu resistance was selected by ultraviolet mutagenesis from the wild type strain gxcr1
用紫外誘變法獲得了gxcr1的銅抗性水平明顯降低的一株突變體mgxcr1 。 -
The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas
對出發菌株ybt - 1463和其無質粒突變株bmb171的部分形態、生理生化和遺傳學特性進行的比較研究的結果表明，突變株bmb171不形成伴胞晶體，但在個體形態與菌落特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發菌株ybt - 1463無明顯差異。 -
Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。 -
In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans, we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining
為了研究耐輻射球菌極強的輻射抗性與dna修復機理，我們應用雙向電泳結合銀染的方法考察了野生型菌株kd8301在射線照射前後細胞體內總蛋白組的變化情況。 -
Plant assay was performed with the wild - type strain gx201, the mutant strain gx217 and the function recovered strain rgx217. the results indicate that the mutant strain gx217 showed a day delay in nodulation time and its abilities of nodule formation efficiency and competitive nodulation were apparently decreased compared with the parental strain gx201 respectively
通過對野生型菌株gx201 ，突變體菌株gx217及其功能互補菌株rgx217進行植株試驗，發現突變體菌株gx217在結瘤時間方面較野生型菌株gx201慢一天，其結瘤效率及競爭結瘤能力均比gx201明顯降低。 -
Secondly another vector phz1117 which for replacement of sc ( j11. 17 / scd78. 01, was constructed. phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained
其次，構建了另一個用於置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3並通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。 -
Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively
隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。 -
However there was not significant difference with respect to the antibiotic production and sporulation from the wild type strain when hxy1 was inoculated on mmasp and r5 medium ; phz1102, a vector with copy number about 10 in streptomyces, was transformed into j1702 and j1700, but it ca n ' t supress the bald phenotype
Hxy1在mmasp及r _ 5平板上培養時，在產素及產孢方面與野生型沒有明顯差異。將phz1102以中等拷貝轉化到j1702和j1700兩種hlda突變株中發現phz1102不能抑制它們的光禿表型。 -
There is no acrystalliferous derivative was screened after treating 4. 5 g / ml ethidium bromide, but after elevating growth temperature of ybt - 1463 and other 8 bacillus thuringiensis parental strains to 42, 9 acrystalliferous derivative were obtained. a series of partially plasmid - cured derivatives were further obtained from 3 acrystalliferous derivatives originated from wild - type strain ybt - 1463 by elevating temperature to 44
用限量培養基和42培養9株野生菌株，結果篩選到9株cry ~ -突變株；繼續升溫至44來培養其中的三個cry ~ -突變株，得到內生質粒被進一步消除的突變株。 -
( 4 ) expressional plasmids containing the cloned pha synthase genes were constructed and transformed into wild type e. coli strains and e. coli fadb deleted mutant strain km32b
（ 4 ）成功構建了含有上述型pha合酶基因的表達載體，並將它們分別轉入野生型大腸桿菌及脂肪酸降解fqdb缺夫突變株km32b中進行表達。
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