Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在對噬菌體環
七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環
七肽克隆展示
肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬
肽。
Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not
篩選tnf小分子模擬
肽及結合
肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合
肽的研究：以rhtnf為靶對噬菌體環
七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短
肽。
After 3 rounds of screening, 10 of 20 clones were identified as positive clones and all the 10 phage clones sharing the same amino acid sequence : ehmaltypfrpp, and these positive 12mer phage clone peptide can bind with tnfa specifically. the results suggests that this method is very specific and simple for screening of binding epitope to small peptide molecule from phage display peptide library
在以噬菌體環七肽tnfa模擬肽克隆刁k naqsoc ）為靶對噬菌體隨機十二肽庫進行h輪篩選后，挑取20個克隆，經elisa鑒定有10個克隆均與t 』 nfa結合，測序結果顯示它們均為同一氨基酸序列： ehmaltypfrpp 。
