參照菌株 的英文怎麼說
中文拼音 [shēnzhàojūnzhū]
參照菌株
英文
reference strain-
Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth
建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken
結果12個參考毒株均能擴增出約679bp的目的片段，而陰性對照的常見5種雞病病原：雞新城疫病毒（ ndv ） 、雞傳染性支氣管炎病毒（ ibv ） 、雞傳染性貧血病毒（ caiv ） 、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段；應用建立的技術對疑似ibd的34份臨床病料進行檢測，並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。
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