噬菌體抗性 的英文怎麼說

中文拼音 [shìjūntǐkàngxìng]
噬菌體抗性 英文
phage resistance
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  1. The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds. the last round enriched phage clones were used to reinfect

    由鼠源噬菌體抗體庫淘選到展示有人絨毛膜促性腺激素單鏈抗體的噬菌體克隆。
  2. Hau3r gene of s. lividans 1326 is directly related to its resistance to actinophage hau3

    野生型變鉛青鏈黴菌1326中的hau3 ~ r基因與1326對噬菌體hau3的抗性直接相關。
  3. When phz2057 was introduced into s. lividans zx1, it could confer partial resistance to actinophage hau3 on s. lividans zx1

    將phz2057轉入變鉛青鏈黴菌zx1 ，能賦予變鉛青鏈黴菌zx1對噬菌體hau3的部分抗性。
  4. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  5. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  6. It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating

    抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。
  7. Amplified in e. coli xi - blue, the eluted phage in the third rounds was poured onto lb / iptg / xgal plate. we selected randomly 18 clones and amplified them, then confirmed positive clones by elisa

    經雙抗體（流感病毒的多抗和辣根過氧化物酶標記m13噬菌體抗體）夾心elisa鑒定的陽性克隆有12個，分別將其純化、並進行dna序列分析。
  8. [ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189, the clones were tested by dot blotting and elisa

    並通過硝酸纖維膜斑點印跡法進行陽性克隆鑒定。 elisa分析陽性噬菌體克隆與抗體的親和力。
  9. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  10. Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library

    從噬菌體抗體庫中淘選人絨毛膜促性腺激素的單鏈抗體
  11. 2. the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv. seven putative epitopes could be recognized by antisera

    利用prrsvbj - 4陽性血清和鼠源抗重組結構蛋白抗體，採用間接elisa方法對噬菌體展示的表位進行了鑒定。
  12. In order to provide valuable data for further exploring the immunogenicity and function of structural proteins of prrsv and designing epitoe vaccine for prrsv, the purpose of the research was to characterize the epitopes on structural proteins of prrsv bj - 4 by means of phage display technique, elisa and gene expression etc. 1. the epitopes and secondary structures of the structural proteins of prrsv bj - 4 were forecasted by molecular biology software goldenkey

    本研究利用噬菌體展示技術，結合elisa和基因表達等技術對prrsv結構蛋白的抗原表位進行了鑒定與分析，為prrsv的結構蛋白免疫特性與功能研究以及表位疫苗的設計等奠定了基礎。
  13. The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm

    首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應用重組噬菌體抗體庫技術，構建了一個針對牛精子的噬菌體抗體文庫。
  14. In this study we screen the phage - epitope library in attempt to predict the receptor recognition site on vegf, identify peptide able to block the interaction of vegf - kdr and find a potent vaccine against tumor angiogenesis by targeting vegf

    [方法]以兩個具有中和vegf活性的抗- vegf單克隆抗體jh121 、 vg189為目標，分別對噬菌體隨機7肽、 12肽庫進行篩選。
  15. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  16. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  17. The antibody induced by peptide gwyydal abolished vegf - induced huvec growth in dose dependent which specifically express the kdr. moreover, both of the peptide gwyydal and vasavfysalve displaying on phage also inhibited the growth of huvec

    [結論]從噬菌體肽庫中篩選到了與抗體有高親和力的陽性噬菌體克隆，噬菌體表達的7 、 12肽gwyydal 、 vasavfysalve模擬了vegf的抗原表位，引起顯著的抗- vegf抗體反應。
  18. Study of immunity of epitope of tumor special antigen displayed on a filamentous bacteriophage

    噬菌體展示腫瘤異性抗原表位的初步免疫活性研究
  19. Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out

    的小鼠噬菌體抗體庫，並從中淘選出對n -肽有結合活性的單鏈抗體。
  20. Pcr detection found there were 1. 2x104 tfu in the library. one of colonies of scfv antibody, which binds to bull sperm, was selected and sequenced. sequence analysis indicates that the scfv colony resembles the mouse antibody

    用phage - elisa從文庫中篩選並鑒定出一個針對牛精子的陽性克隆，序列分析發現，該單鏈抗體克隆的序列符合小鼠抗體可變區的一般特徵，這說明構建的針對牛精子的噬菌體抗體文庫是成功的。
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