實用生物學 的英文怎麼說
中文拼音 [shíyòngshēngwùxué]
實用生物學
英文
department of applied biology- 實 : Ⅰ形容詞1 (內部完全填滿 沒有空隙) solid 2 (真實; 實在) true; real; honest Ⅱ名詞1 (實際; 事實...
- 用 : Ⅰ動詞1 (使用) use; employ; apply 2 (多用於否定: 需要) need 3 (敬辭: 吃; 喝) eat; drink Ⅱ名...
- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 學 : Ⅰ動詞1 (學習) study; learn 2 (模仿) imitate; mimic Ⅱ名詞1 (學問) learning; knowledge 2 (學...
- 生物學 : biology
- 生物 : living things; living beings; organisms; bios (pl bioi bioses); biont; thing; life生物材料 biol...
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It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證Thirdly, resorting to cooperation - competition model of biomathematics, this thesis proposes a new co - evolution model. simulation results are shown to verify its effect and practicabilitv. last, standard methods for optimizing neural netvvorks are easily trapped into local optimization, and unable to adjust the structure of neural networks, thus their application is limited to certain extent
本文主要驗證和設計適應性操作運算元和小生境方法保持群體多樣性的能力,實驗表明兩種方法都能較好地達到目的;利用生物合作競爭模型設計協同演化來動態地改變群體規模,實驗表明該模型是有效的;人工神經網路是得到越來越廣泛應用的學習系統,但是由於學習演算法存在一定的缺陷,如易於陷入局部極值,難以調整網路的結構等,使神經網路的應用受到一定的限制。It can be apply widely to the research of plant physiology, bionomy, agriculture, forestry and molecule biology etc. the system makes use of principal and subordinate mode virtual instrument theory
可以滿足大學本科和研究生有關實驗課教學及研究要求,可以廣泛應用於植物生理學、生態學、農學、林學和分子生物學等學科的研究中。Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way
由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化,以原生質體作受體是蘚類植物轉化的常用途徑。In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。On the basis of foreign documents, the dissertation firstly describes the morphology, taxonomy, and biological characters of cochineal insects. then, by using artificial climate cases in the lab, according to orthogonal design, 8 lands of combinations of temperature, humidity and light is set, while the temperature has four levels : 15, 20, 25 and 30, and the relative humidity has two levels : 60 % and 80 %, while the light also has two levels : 980 lux and 60 lux. through observations and analysis of hatching percentage, survival percentage of the first instar and during from the second instar to the adult, the population tendency index, generation life cycle, size and eggs of female adult, the development of the cochineal under different conditions is studied
在收集、整理國外對胭脂蟲的研究資料基礎上,首先從胭脂蟲的形態學、分類學、生物學特性入手,進而在實驗室內利用人工氣候箱,應用正交設計方法,設計了4種溫度、 2種濕度、 2種光照梯度的不同組合,通過測定胭脂蟲的孵化率、 1齡若蟲的存活率、 2齡若蟲至成蟲期存活率、種群趨勢指數、世代歷期、雌成蟲的大小、懷卵量等生物學指標,深入研究胭脂蟲在這些組合下的生長發育狀況,應用數理統計方法,分析出溫度、濕度和光照對各項生物學指標的影響,找出胭脂蟲適宜的室內培育條件,並為野外胭脂蟲的培育提供一定的理論依據。From different points of view of the epidemiology, cytogenetics, molecular biology, experimental pathology and preventive medicine, this study revealed and proved that the genotoxicity and carcinogenesis existed in the drinking pond water in the high incidence area of live cancer in guangxi, indicated the feasible preventive and interruptive methods, too
從分析流行病學,細胞遺傳毒理學、分子生物學、實驗病理學和預防醫學的不同角度,揭示和論證了廣西某肝癌高發區居民飲用塘水的基因毒性和致癌性,提出了切實可行的預防和阻斷措施。Exploring the possible mechanisms is an important content of bioinorganic chemistry and ecotoxicology, which has signality in modifying contamination effect to animals. this thesis studies on the chemical speciation of mercury and selenium in dolphin livers, sub - chronic toxic effect of wistar rats of sodium selenite and mercuric chloride and investigates the antagonism mechanism from there aspects : 1. redistribution of mercury in the presence of selenium, 2
本文以海豚肝臟為對象,研究其積累的高濃度汞硒化合物的化學形態和結構,並結合動物實驗的研究,從汞硒相互作用的總體生物學表現,汞硒在動物體內分佈變化和結合形態的變化以及汞硒化合物的結構等方面探討兩元素毒性拮抗作用機理。In external studies of mechanism may be a feasible approach to explore biological effects of rf radiation in the case of no persuadable conclusion of epidemiological and in external studies
因此,盡早明確射頻場的作用機制從而確定流行病學和實驗研究的生物指標,是揭示移動電話射頻輻射生物學效應的必要途徑。The study was primarily conducted to investigate the effects of various irrigation managements on the yield, quality and wue of alfalfa and the sustainable utilization of soil - water in north china plain. five treatments were applied : 0mm, 30mm, 50mm, 75mm and 100mm irrigating at the beginning of the second growth cycle in 2001
本研究通過兩年的灌溉實驗,根據對降水情況、土壤水分的蒸發量、蒸散量與苜蓿各生物學指標的分析,初步探討了在華北平原地區不同灌溉處理對于苜蓿產量、品質、水分利用率以及苜蓿地土壤水資源利用可持續性的關系。The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again
應用間接免疫熒光試驗進行血清學鑒定,結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序,在genbank上進行同源性比較,發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高,分別為94 、 95 ,從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上,本實驗對兩株病毒進行了核苷酸全序列測定。His most famous experiments, begun in 1889, demonstrated the conditioned and unconditioned reflexes in dogs, and they had an influence on the development of physiologically orientated behaviourist theories of psychology during the early years of the 20th century
他很多著名的實驗,開始於1889年,他用狗來做有條件反射和無條件反射的實驗,這對生物學的發展有很大的影響,同時對二十世紀初的心理學理論發展方向有一定的影響作用。Abstract : in this paper interactions of bilayer lipid membrane with clotrimazole as the antifungal agent were reported in order to interpret the action basis about the molecularbiology of this drug. using the injecting method , we prepared three bilayer lipid membranes ( blm ) respectively, and by the cyclic voltammetry method we determined properties of blm before and after adding clotrimazole into the membranes. it was found that clotrimazole could remarkably reduce the membrane resistance and demonstrated that the drug as an antifungal substance might certainly change the permeability of bilayer lipid membrane
文摘:報道了抗真菌藥克霉唑與人工脂膜的相互作用,試圖闡明該藥的分子生物學的作用基礎.用注射方法分別制備3種雙分子層脂膜( blm ) ,並運用循環伏安法測量了修飾物克霉唑加入前後人工雙分子層脂膜的電性質.結果發現克霉唑可顯著降低膜電阻並證實該藥確能改變人工雙分子層脂膜的通透性Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine
在簡要介紹結構生物學的研究方法的基礎上,主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系,通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等,以及結構生物學在醫學上的應用。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外( invitro )實驗結果,為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據,根據動物中的一些研究方法,本實驗設計並構建了帶有信號肽、 cam結合肽( can小肽) 、 epitope ( c - myc )融合基因的載體,並將融合基因通過真空滲入法轉入擬南芥,預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合,這樣就會抑制質外體cam的功能,從而可以構建質外體cam的「反義」植株,通過觀察質外體cam 「反義植株」的表型改變,就可以推斷質外體cam在植物生長發育過程中的功能。Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal
本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究,並與生化位點標記分析法進行比較,旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法,為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。The technique of insect cell culture, one of the bases of cell engineering, is a valuable method of modern experiment biology
昆蟲細胞培養技術作為細胞工程基礎之一,是現代實驗生物學上極有價值的手段之一,廣泛應用於生物學、醫學及農業的各個領域。First, features of ncrna were summed up to serve as input for data mining procedures later on. second, statistics toolbox and artificial neural networks toolbox of matlab were used to carry out principal components analysis and artificial neural network training. finally, user prediction was designed in visual c + + while matcom served as interface between matlab and vc to complete the user prediction program
首先利用生物學實驗數據總結出ncrna的特徵,作為數據挖掘方法的輸入;然後在matlab環境下用統計工具箱和神經網路工具箱對輸入的特徵進行主成分分析和神經網路訓練,用訓練好的網路去預測ncrna ;最後,為了實現通用性,運用matcom介面與vc實現windows下供用戶實際使用的預測程序。分享友人