導出水平片段 的英文怎麼說
中文拼音 [dǎochūshuǐpíngpiānduàn]
導出水平片段
英文
derived horizontal fragmentation- 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
- 水 : 名詞1 （由兩個氫原子和一個氧原子結合而成的液體） water 2 （河流） river 3 （指江、河、湖、海、洋...
- 平 : Ⅰ形容詞1 （沒有高低凹凸 不頃斜） flat; level; even; smooth 2 （高度相同; 不相上下） on the same l...
- 片 : 片構詞成分。
- 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
- 導出 : leading-out; derive; derivation
- 水平 : 1. （跟水面平行的） horizontal;level2. （達到的高度） standard; level
- 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
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The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。Based on these results, the strategies for conservation e. mollis population to prevent the isolation and fragment of the populations and maintain the high genetic variation among the populations. moreover, it should be avoid the decline of gene flow was resulted from the isolation of the populations so that resulted in the differentiation and genetic decline of the population. finally, it should be adopt some method to spread continuous distribution and extend genetic diversity of the population
針對翅果油樹的遺傳結構和遺傳多樣性的研究結果，提出了保護翅果油樹種群的策略：維持現有翅果油樹種群高的種內遺傳變異水平，遏制由於人為原因而導致的翅果油樹種群隔離或片段化的趨勢，防止由於種群隔離造成基因流降低，從而引起種群分化和種群的遺傳衰退，並採取適當措施擴大種群的連續性和種群內的遺傳多樣性水平。Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
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