嵌合體鼠 的英文怎麼說
中文拼音 [qiàngětǐshǔ]
嵌合體鼠
英文
chimera mouse-
To allow these studies, researchers have developed chimeras
正是為了使這方面的研究成為可能,人鼠嵌合體應運而生。The application of dna fingerprint technique in monitoring the es cell chimeric mice
Dna指紋圖在小鼠es細胞嵌合體鑒定中的應用Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。After renaturation, the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however, hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation
復性后的mv a摘要生物學活性接近天然mvlla的100 % ;嵌合體hm一1227的活性小於hwtx一i的15 % ;而嵌合體fd以一xw不能阻斷小鼠隔神經一隔肌的接頭傳遞。Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術,檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。The experiment includes two procedure. first, sd rats were conditioned with 5. 5gy sublethal total body irradiation ( tbi ), followed by infusion of balb / c mice bone marrow cells 8x107 on day 0, then were intraperitonially administered cyclophosphamide ( cp ) 150mg / kg on day 2. the procedure let sd rats become chimerism and have specific immunologic tolerance
實驗分兩步:第一步, sd大鼠經亞至死劑量5 . 5gy全身照射后,尾靜脈輸入balb c小鼠的骨髓細胞8 10 ~ 7 , 2天後腹腔內注射環磷酰胺150mg kg ,誘導形成嵌合體大鼠,使其對balb c小鼠產生特異性免疫耐受。Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的:以il - 12作為分子佐劑,與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子,將其克隆到真核表達質粒中,構建成嵌合dna疫苗,研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用,為尋求安全、有效、廉價的結核病新疫苗打下基礎。The results showed that es cells derived from kunming albino mouse remained undifferentiated state when they were cultured in vitro for a long time, and karyotypies were stable, but the ability of germ - transmission have not been verified
結果顯示,用昆明白小鼠囊胚所建立的胚胎幹細胞在體外長期培養時仍能保持未分化的狀態,核型穩定,但還未證明其具有生殖系嵌合的能力。One chimaeric mouse was produced by injection of es cells into blastocyst of c57bl / 6j. the chimaeric rate of fur colour was about 15 %. fl generations of germ - transmission derived from es cell were not obtained when chimaeric mouse mated with kunming albino mice and c57bl / 6j mice
將胚胎幹細胞注射到c57bl 6j小鼠的囊胚腔內,移植后產生了一隻wk2 -胚胎幹細胞的雄性嵌合體鼠,毛色嵌合率為15左右,成熟後分別與c56bl 6j和昆明白雌鼠交配,均未得到來自es細胞的後代。This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability, then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection. finally, first chimeric pig in china was delivered, and by chimera analysis, chimerism is present in some tissues
本研究首先利用近交系昆明白小鼠進行胚胎顯微注射觀察發育效果,然後利用所獲五指山豬eg細胞製作嵌合體檢驗eg細胞多能性,最終獲得國內首例胚胎幹細胞( eg細胞)嵌合體豬,經dna分析確認在多種組織中存在嵌合狀態。Based on the research of the performance, resource and exploit of the stromgarm cpu, general scheme of media control board of videophone based on embedded microprocessor strongarm and emebedded operating system windows ce is designed. it performs the customization of embedded operating system, and designs and develops the programs of system start - up, the driver of color lcd, standard rs232 serial port, serial mouse, ethernet boot loader, and so on
結合系統所使用的stongarmcpu分析了它的性能、資源、功耗等進行了分析,提出了基於嵌入式微處理器strongarm和嵌入式操作系統windowsce的可視電話的多媒體控制板的總體方案,對嵌入式windowsce進行了平臺定製,設計和開發了系統引導程序、彩色lcd 、標準r232串口、串口鼠標、 ethernetbootloader等驅動程序。分享友人