巰基蛋白酶 的英文怎麼說
中文拼音 [qiújīdànbái]
巰基蛋白酶
英文
thiol protease-
Cysteine proteinase inhibitor, cpi
植物巰基蛋白酶抑制劑Circular dichroism studies of cysteine proteinase inhibitor from rice
水稻巰基蛋白酶抑制劑的圓二色譜研究The changes of these endopeptidase are different during the former vasing of " samatha " and " belami ". the main eps in petals of " samatha " are the acid thiol - proteinase, metallo - proteinase and the alkali serine proteinase and the main eps in petals of " belami " are the acid thiol - proteinase and serine proteinase. during the later vasing the acid metallo - proteinase and the alkali serine proteinase become main eps in petals of " samatha " and " belami "
這3種內肽酶在兩種切花瓶插初期（盛開以前）的表現不同： 『薩蔓莎』花瓣中以酸性金屬蛋白酶、巰基蛋白酶和堿性絲氨酸蛋白酶起主要作用， 『貝拉米』花瓣以酸性巰基蛋白酶、絲氨酸蛋白酶起主要作用，後期『薩蔓莎』和『貝拉米』花瓣均以酸性金屬蛋白酶、堿性絲氨酸蛋白酶起主要作用。Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工酶的sds - page和sds -毛細管電泳（ ce - sds ）顯示，該酶除了含40kd主帶外，還有很淺的66kd帶，和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似； sos - page和ce - sds電泳中，無論加入-巰基乙醇與否， go同工酶都只有40kd主帶和66kd淺帶，表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連；用制備性sds - page法獲得菜心go同工酶的40kd亞基，並和菜心go同工酶一起測定其氨基酸組成，該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ，表明40kd亞基可能是個酸性蛋白，而66kd帶則是個堿性蛋白。There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽酶：巰基蛋白酶、金屬蛋白酶、絲氨酸蛋白酶，三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置，約為52kd ；前兩種最適ph為6 ，是酸性內肽酶，后一種有酸（ ph6 ） 、堿（ ph10 ）兩種存在形式，既是酸性內肽酶又是堿性內肽酶。These proteases were different with the ones reported in past and it suggests that there may be more proteases associated in psii of chloroplasts of spinach. such as, one of them could be resistant to 1 mol / l nacl and be almost inhibited by 1 mmol / l dtt or mercaptoethanol
比如，一種蛋白酶對1mol l濃度的nacl溶液不敏感，但是受到dtt和巰基乙醇的強烈抑制以及1mmol ledta的部分抑制，而且這種蛋白酶能夠降解18kd外周多肽，在較廣的ph值范圍內都具有活性。分享友人