幾丁質化的 的英文怎麼說
中文拼音 [jīdīngzhíhuàde]
幾丁質化的
英文
chitinzed-
The degradation of chitin by chitinase was vital step in the life cycle of insects because insects undergo periodic ecdysis and metamorphosis
幾丁質代謝隨昆蟲不同生長發育階段而變化並保持相對的穩定,對昆蟲的正常的生長發育是至關重要的。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Purification and characterization of a chitinase from endophytic chaetomium spirale nd
35幾丁質酶的純化和性質Chitin ' s partially deacetylated product is chitosan. unlike these polysaccharides, chitooligosaccharide and oligochitosan, which are the oligomer homologs of chitin or chitosan, are soluble and can be satisfactorily utilized
幾丁質部分脫乙酰化產物是殼聚糖,它們的降解產物為幾丁寡糖、殼寡糖,具有較好的水溶性,易於分散吸收。Zhang xue - kun, tang zhang - lin, chen li et al. effect of partially n - acetylate chitosans to elicit resistance reaction on brassica napus l., agricultural sciences in china, 2002, vol2 ( 3 )
張學昆,唐章林,諶利,李迦納等.脫乙酰化幾丁質乙酰化程度對油菜抗性的影響.中國農業科學, 2002 ( 3 )Producing plant phytoalexins has been recognized as the primary and dominant routines in defending system in cotton, include terpenoid, proteases and hormones in which many enzymes, such as chitinase, has been proved useful against verti - cillium in several plants, but not their functional mechanism
在棉花抗病反應中以植物抗毒素的合成為主要途徑,包括萜類、酶類和激素類3類化學物質,其中酶類物質如幾丁質酶已經在很多植物中被證明具有抗病作用,但是其具體作用途徑仍不清楚。Chi 1 degraded colloidal chitin both endolytically and exolytically with jv - acetylglucosamine, chitobiose and chitotriose as its products while a c - teminal 302 - amino - acid d eleted mutant had its degrading pattern shift to chitobiose, chitotriose and chitotetraose. further research showed a shifting of the minimal length of chitooligosaccharide needed for these two correlated proteins. in the case of full - length chitinase, it was chitotriose while with its truncated form, it turned out to be chitotetraose
進而對chi1和chi1 ~ -做了體外表達、純化及功能的比較分析,首次揭示了幾丁質酶潛在的幾丁結合區對酶作用底物與產物的特異性影響,即chi1 ~ -仍具有較強活性,但其膠體幾丁質降解產物為幾丁二、三、四糖。But high insolubility restrict its application, so we need to carry on chemical milling to it, carry on the structure and decorate or degrade its strand partly
但是,幾丁質不溶於水限制了它的應用,往往需要對它進行一些化學修飾或部分降解以增加其溶解性。Screening and purification of neutral protease with chitinase inhibitor activity
具幾丁質酶抑制活性的中性蛋白酶的篩選與純化Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained
用ecori和sall對含有幾丁質酶基因的重組質粒tvchi (含幾丁質酶編碼基因的pmd18一t載體)和穿梭質粒phy300plk進行雙酶切並連接,轉化e . colidhs 。The large - scale expression product is concentrated and desalted by ultra - filter, and the target protein is separated by affinity chromatography with chitin, and then cut by cnbr. the purified product appears evident antibacterial activity with method of agar diffusion
採用優化的表達條件進行大量發酵,對上清液超濾濃縮脫鹽后,採用幾丁質基質進行親和層析分離目的蛋白,溴化氰切割,瓊脂孔穴法測抗菌活性。A recombinant expression plasmid was first constructed by inserting the vitreoscilla hemoglobin gene ( vgb ) into the plasmid pchi, which contains the chitinase gene cloned from bacillu circulans c - 2. to study the effect of vhb on cell growth and chitinase production, the expression plasmid was transformed into escherichia coli jm109
為了進一步提高幾丁酶基因在大腸桿菌的表達,將透明顫菌血紅蛋白基因( vgb )插入到幾丁酶基因表達質粒pchi后轉入大腸桿菌jm109中, vgb基因的表達促進了轉化菌的生長和提高了幾丁酶基因的表達,溶氧水平越低,作用越顯著。分享友人