引誘物測定 的英文怎麼說
中文拼音 [yǐnyòuwùcèdìng]
引誘物測定
英文
bioassay of attrac-tants- 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
- 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
- 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
- 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
- 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
- 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
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On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
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