拷貝序列 的英文怎麼說
中文拼音 [kǎobèixùliè]
拷貝序列
英文
copy sequence-
1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。The genomic osbhlhl gene is a unique copy gene in rice genome with four introns
Osbhlh1基因的在基因組序列中是單拷貝的，有4個內含子。All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function
本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis. we took a pcr method to clone the cdna of osdd2 gene
使用克隆的邊端插入序列為探針對水稻（中花11品種）進行southern雜交分析，表明osdd2基因在水稻基因組中是以單拷貝存在。In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Results : designed and synthesized the coding gene of echistatin. and constructe its single copy expression vector, identify the vector by dna sequencing. but it could not express echistatin in e. coli. designed and synthesized the modified coding dna of echistatin, and constructe its two copy expression vector, identify the vector by dna sequencing, but it could not express echistatin in e. coli
結果設計併合成了echistatin編碼基因dna序列，構建了echistatin單拷貝表達載體，經dna測序鑒定正確后， sds - page及western - blotting結果表明： echistatin在上述菌株中未獲得高效表達。In the first part of this experiment, we isolated the tsmt gene ( genebank accession number : af499726. 1 ) from a a zap - cdna library cons tructed from a 200mmoll - 1 nacl - treated library of thellungiella salsuginea and analyzed its sequence characterization, genomic organization and the differential expression in response to salt stress. tsmt encodes metallothionein and there is only one copy in thellungiella salsuginea genome according to its southern blotting
L ~ （ - 1 ） nacl處理的鹽芥地上部分構建的zap - cdna文庫中克隆了編碼金屬硫蛋白的基因tsmt （ genebank號為af499726 . 1 ） ，並分別對其序列特徵、基因組結構和在鹽脅迫下的表達特性進行了分析，結果表明tsmt編碼鹽芥金屬硫蛋白， southern結果顯示tsmt在鹽芥基因組中只有一個拷貝。Sequencing requires multiplecloned copies of the gene ; long dna sequences are cut into more manageable lengths using restriction enzymes
序列需要基因的多個克隆拷貝，用限制性酶可以把長的dna序列切成許多具所需長度的片段。Transgenes should be with single copy and without accessory sequence, and with consistence to express and stable genetic in various transgenic plants in a new gene transformation program
新的轉化程序要求把基因導入農藝性狀優良的品種中，呈單拷貝、不帶有輔助序列，並在不同的轉化體中表達一致，穩定遺傳。分享友人