接合后體 的英文怎麼說
中文拼音 [jiēgěhòutǐ]
接合后體
英文
exconjugant-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The necessary expenses for the remains repatriation service include the expenses for embalming, preserving, cremating, transporting, cinerary casket and other materials and services, which will be paid by the company after verified directly to international sos, but subject to a maximum of the basic sum assured as stated in the rider
遺體送返服務所需的費用經本公司核實確認后直接支付給美國國際支援服務公司,費用總數最高以投保單或批註上所載的本附加合同基本保險金額為最高限額。Tensile properties and impact properties measurements were done at room temperature. the volume resistivity of rectangular samples was measured using a zc36 electrometer and a high 240a voltage supply, for samples with a low resistivity level a dt - 9205b digital multimeter was used, silver paint was applied to ensure complete contact between sample and electrodes, namely, to eliminate the contact resistance. the phase morphology of blends was also studied using a jeol jsm - 5900lv scanning electron microscope ( sem )
體積電阻率的測定:當r _ v 10 ~ 8時,製成100 100 4mm板材,用zc36型高阻儀測量;當r _ v 10 ~ 8時,用dt - 9205b型數字式萬用表測試試樣的體積電阻,為了減小接觸電阻對測試的影響,採用銀導電膠將銅片粘接在試樣的兩個端面上,靜置24小時,待銀導電膠凝固,試謝長瓊:熱拉仲對pet / pe / cb復合導電體系形態和性能的影響樣的電阻穩定后再測量。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。He said using un personnel as human shields is " a favorite trick by people who don ' t have representation in the un, " knowing they will suffer no direct consequences for the violation
將軍同時還說,把聯合國人員作為人體盾牌已成為「那些在聯合國沒有代表的人們的最熱衷的一個竅門」 ,因為他們知道這樣做,可以在實施攻擊后免受直接的後果(還擊) 。According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions
在維他命b12的吸收方面,根據醫學報導,維他命b12在吃進人體后,首先會在胃部,和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合,形成復合物后,再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時,需要有鈣離子之存在。In our future real right law, there should be some limitations to the open objects, hut nor the open contents in the property register of immovables ; lawsuit time limit should not he applied to return protoplast right of applicant ; we had better constitute positive prescription system ; powerless punish should not affect the force of contracts when the endorser has no right, but the transferee is well - meaning ; there should be proper toleration to the unanimous consent principle on punishing mutual thing ; we should prohibit mortgaging to some movable property which has no way to open ; there should not be the time limitation when mortgager realizes the hypothec after the fulfilling tern ; of primary creditor ' s rights is over, except that mortgager is not the debtor ; when the debtor do not refund the debt, the mortgager cannot get the guaranty directly, but he may put in for the court to auction guaranty
摘要我國未來物權法,對于不動產登記簿的開放對象應當有所限制,但對于開放內容不應有所限制;物權人的返還原物請求權不應當適用訴訟時效;應當建立取得時效制度,並應區分一般動產、準不動產、未登記不動產而規定不同的成立條件;在動產的轉讓人為無權處分而受讓人為善意的情況下,轉讓人與受讓人之間的合同是否有效不應當以「無權處分」為條件,只有在轉讓合同無效的情形下,受讓人取得標的物的所有權才是依善意取得制度的取得,此時的取得是原始取得;在共同共有的情形下,原則上處分共有物應當經共有人全體一致同意,但應容有若干例外;不應當規定居住權;動產抵押的公示方式問題無法徹底解決,如果規定動產抵押,應當實行登記要件主義,適于烙印、打刻或貼標簽的動產,應當採取烙印、打刻或貼標簽的方式,其他無法解決公示方式的動產應當禁止抵押;主債權履行期屆滿后,抵押權人行使抵押權,原則上不應當有一個時間限制,但抵押人非為債務人時可容有例外;在債務人不償債時,抵押權人不能直接讓抵押人交出抵押物,實現抵押權,但可以持抵押權登記簿副本直接申請法院拍賣抵押物。The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板,加入制備好的肽庫,用tbst洗去非特異結合的噬菌體,加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ,室溫放置10min以洗脫特異結合的噬菌體, 2mol ltris - hcl ( ph9 . 1 )中和后,取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定,其餘噬菌體擴增後用于下一輪篩選,共重復3輪淘洗。After renaturation, the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however, hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation
復性后的mv a摘要生物學活性接近天然mvlla的100 % ;嵌合體hm一1227的活性小於hwtx一i的15 % ;而嵌合體fd以一xw不能阻斷小鼠隔神經一隔肌的接頭傳遞。When the solar system was newly formed, the huge gravity of jupiter pulled planetesimals away from the sun, indirectly putting the earth under a collision barrage of objects of various sizes during the first hundred millions of years after the formation of the earth. hese violent impacts increase the amount of basic elements of life, water and organic compounds, on earth
在太陽系形成之初,木星巨大的重力把組成行星的物質牽引離開太陽,間接令地球在它形成后的最初數億年間,飽受不同大小的物體撞擊,這些猛烈的撞擊為地球增添了不少構成生命的重要元素水和其它有機化合物。The bulk of the mass is spread out, forcing stars to trace a spirograph - like rosette : an ellipse that does not close on itself but gets offset each time the star completes one revolution [ see box at right ]
整體質量分散開來,迫使恆星運行方式如旋轉繪圖盤所畫出的花瓣狀螺線:那不是每圈都會封閉起來的橢圓,恆星繞行一周后,軌道並不接合便又重新開始下一周的運動(見右方整理軌道秩序) 。Along with the technology mature in the soft switching and media gateway, the narrow band access equipment will be integrated the function of media gateway. users may access directly to the broadband ip man through twisted cabling or wireless base station. dial - up server will also be replaced by the media gateway, narrow band dial - up users will be diverted to broadband ip man at the access network
隨著軟交換和媒體網關技術的成熟,在窄帶接入層設備上將綜合媒體網關的功能,用戶通過雙絞線或無線基站接入后,可以通過媒體網關直接接入寬帶ip城域網,撥號服務器也將大量被媒體網關取代,窄帶用戶撥號上網將在接入網被分流至寬帶ip城域網。An auxiliary switch is added, it is integrated with handler of the isolation switch, grounding switch. the maintenance for the auxiliary switch can be carried out in the maintenance room. there are interlock between the door and isolation switch or the grounding switch, only after the isolation switch is open or the grounding switch is closed, the door can be opened, it is the unique characteristics of the substation
另外我們生產的智能型箱式開關站採用了強制性的jsxgn型的機械閉鎖;為了簡化結構,提高可靠性,也對其進行了設計改進:增加了輔助開關,使其與隔離開關、接地開關的操作手柄為一體,並可在維護走廊對輔助開關進行檢修;箱體的門板與隔離開關或接地開關之間也有連鎖,只能在隔離開關打開后或接地開關閉合后,才能被打開;此結構也是本開關站所獨有。The ever - improving devices and facilities, perfect services especially high - standard commercial services including computer network facilities can well satisfy customers needs in board, lodging, transportation, tourism, shopping, recreation, commercial activities, conference etc. in the year of 1997, a re - investment for tens of millions rmb was realized to carry out decorations and renovations to the guestrooms, reception hall and other hardware of the hotel so as to make the hotel meet the national standard of a five - star hotel. de - luxe commercial floors and supplementary installations were made available. fuzhou lakeside hotel, resplendently and gorgeously renovated, is embodied with rich cultural environment and fresh up - to - date quality and is impressively true to the reputation of the top building by the side of beautiful fuzhou west lake
回想西酒開業初期,這家省政府屬下的中外合資企業在香港人掌管下也曾歲月蹉跎,然而,年輕的中方領導全面接管后的酒店,面對外界的種種疑慮,先後兩任總經理帶領全體員工,發奮圖強,發揚"敬業求實互助賓客友人,發揮了對外開放重要的視窗作用,令人信服的數字連續三年營業額超億元和一項項榮譽兩年全國五十家最佳星級飯店全國衛生先進單位等記載了酒店不斷前進的腳步,奠定了酒店在福建省旅遊業中的頭地位。The phytic acid has the function of complexing with metal. it easily forms a protection film of single molecular on the metal surface to obscure the contact of oxygen with the metal and prevent magnesium alloy from corrosion in the process of electroplating
添加劑植酸與金屬配合后,易在金屬表面形成一層緻密的單分子保護膜,能有效地阻止氧氣與金屬基體接觸,從而可以防止鎂合金基體在電鍍過程中的腐蝕。As cyclin b is the mark protein representing the process of prophase, we infered that cytochalasin b destroyed the polymerization of the nuclear actin and directly affected the process of prophase. nuclear actin played an important role in the process of the chromosome construction
因為cyclinb是有絲分裂前期進程的標志性蛋白,所以我們認為細胞鬆弛素b破壞細胞核內肌動蛋白聚合后,直接影響了有絲分裂前期的進程,進而悅明核內肌動蛋白在有絲分裂前期染色體構建過程中起著重要作用。Compared with uncoated graphite, contact angle with water of all of the composite particles are much smaller and decreased gradually with the increase of the coating amount
包覆后,各類復合粉體與水的接觸角都比未處理石墨低,而且隨著包覆率增大,接觸角有進一步降低的趨勢。On the base of least squares, using measured data and some optimization methods, both bdm and sfm can get the simulation of stress field and directly provide initial stress state for subsequent stability analysis of slope. bdm can overcome the shortcoming of excess assumption resultiag from other simulation methods, and be widely used in elastic - plastic model. 4
這兩種方法是利用現有的實測數據,基於最小二乘法制定目標函數,採用一定的優化演算法,結合有限元分析程序,得到巖體初始地應力場,並直接為后續的邊坡穩定性分析提供初始應力狀態。When connector is totally mated, convex platform of pin is fully fitted with horn mouth of socked insert in order to protect it from dust, liquid and harmful gas
當連接器完全插合后,插針的1臺與插也安裝板的喇叭完全吻合,可心目外界的灰塵、液體、有害氣體的侵入。Second, on the base of large scale integer building model tests, anti - seismic property of structure of multi - rib panel is analyzed, and is contrasted with the property of the masonry structure. the research reveals that buildings in structure of multi - rib panel make shear deformation mainly, whose failure process is similar to the single composite panel and the tests result shows that, contracted to masonry structure, carrying capacity of buildings in this structure is 1. 5 times and deformation capacity is about 3 times. in addition, thanks to the lower self - weight, the seismic response of the buildings is far lower than masonry structure
研究表明:多層密肋壁板結構房屋以剪切變形為主,破壞過程與單塊墻板相似;復合墻板與后澆隱型框架連接成整體結構,協同工作;傳統的底部剪力法適用於本結構體系的抗震計算;試驗結果還表明,該結構房屋最大承載能力是磚混結構的1 . 5倍,變形能力是磚混結構的3倍左右,而房屋自重比砌體結構減少1 4以上,地震反應遠低於砌體結構。分享友人